Microfluidic system for monitoring temporal variations of hemorheological properties and platelet adhesion in LPS-injected rats

Scientific Reports
Eunseop YeomSang-Joon Lee

Abstract

Sepsis causes multiple organs failures and eventually death. Changes in blood constituents due to sepsis lead to alterations in hemorheological properties, and cell adhesiveness. In this study, a new microfluidic system is proposed to measure temporal variations in biophysical properties of blood after injecting lipopolysaccharide (LPS) into a rat extracorporeal model under ex vivo condition. To measure blood viscosity, the interfacial line between blood and a reference fluid is formed in a Y-shaped channel. Based on the relation between interfacial width and pressure ratio, the temporal variation in blood viscosity is estimated. Optical images of blood flows are analyzed by decreasing flow rate for examination of red blood cell (RBC) aggregation. Platelets initiated by shear acceleration around the stenosis adhere to the post-stenosed region. By applying a correlation map that visualizes the decorrelation of the streaming blood flow, the area of adhered platelets can be quantitatively attained without labeling of platelets. To assess sepsis inflammation, conventional biomarkers (PCT and IL-8) are also monitored. The increasing tendency for blood viscosity, RBC aggregation, platelet adhesion, and septic biomarkers are observed ...Continue Reading

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Citations

May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rui GuoWeiying Lin
Mar 15, 2019·Biomicrofluidics·Hyeonji HongEunseop Yeom
Oct 16, 2018·Clinical Hemorheology and Microcirculation·Eunji KoChoon Hak Lim
Dec 26, 2018·Clinical Hemorheology and Microcirculation·Hoyoon LeeSehyun Shin
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Mohammad Mohammadi AriaOzlem Yalcin

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Methods Mentioned

BETA
ESR
light scattering
ELISA

Software Mentioned

Matlab
MARS Data Analysis

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