Microfluidics analysis of red blood cell membrane viscoelasticity

Lab on a Chip
Giovanna TomaiuoloStefano Guido

Abstract

In this work, a microfluidic system to investigate the flow behavior of red blood cells in a microcirculation-mimicking network of PDMS microchannels with thickness comparable to cell size is presented. We provide the first quantitative description of cell velocity and shape as a function of the applied pressure drop in such devices. Based on these results, a novel methodology to measure cell membrane viscoelastic properties in converging/diverging flow is developed, and the results are in good agreement with data from the literature. In particular, in the diverging channel the effect of RBC surface viscosity is dominant with respect to shear elasticity. Possible applications include measurements of cell deformability in pathological samples, where reliable methods are still lacking.

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Citations

Oct 9, 2014·BioMed Research International·Judith C A CluitmansGiel J C G M Bosman
Feb 27, 2013·Lab on a Chip·Kevin B RothDavid W M Marr
May 15, 2012·Lab on a Chip·Yi ZhengYu Sun
Jun 15, 2015·Medical Engineering & Physics·Giovanna TomaiuoloStefano Guido
Dec 15, 2015·Medical Engineering & Physics·Rosa D'ApolitoStefano Guido
Mar 15, 2011·Microvascular Research·Giovanna Tomaiuolo, Stefano Guido
Jan 31, 2015·Advances in Colloid and Interface Science·A PerazzoStefano Guido
Sep 19, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rosa D'ApolitoStefano Guido
Mar 25, 2014·IEEE Transactions on Bio-medical Engineering·Chia-Hung Dylan TsaiMakoto Kaneko
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Dec 20, 2011·Lab on a Chip·Kwang W OhEdward P Furlani
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Aug 23, 2018·Lab on a Chip·Azeem AhmadBalpreet Singh Ahluwalia
Apr 12, 2019·Soft Matter·Margaret G O'ConnellSujit S Datta
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May 23, 2019·Lab on a Chip·Francesca SorbaCristina Martin-Olmos
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Apr 9, 2019·Journal of the Royal Society, Interface·Viola IntroiniStefano Guido
Mar 16, 2019·The European Physical Journal. E, Soft Matter·Rosa D'ApolitoStefano Guido
Jun 3, 2021·International Journal of Molecular Sciences·Giulia Della Pelle, Nina Kostevšek

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