Microfluidics-based on-a-chip systems for isolating and analysing extracellular vesicles

Journal of Extracellular Vesicles
Shang-Chun GuoHelen Dawn

Abstract

Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated "on-a-chip" systems. In addition, this review also discusses the pote...Continue Reading

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Methods Mentioned

BETA
biopsy
density gradient centrifugation
membrane filtration
fluorescence imaging
electrophoresis
nuclear magnetic resonance
chip
enzyme-linked immunosorbent assay
biosensor
ELISA

Software Mentioned

Exodisc
Exos
ExoTIC
ExoChip

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