Microfluidics within a well: an injection-molded plastic array 3D culture platform

Lab on a Chip
Younggyun LeeNoo Li Jeon

Abstract

Polydimethylsiloxane (PDMS) has been widely used in fabricating microfluidic devices for prototyping and proof-of-concept experiments. Due to several material limitations, PDMS has not been widely adopted for commercial applications that require large-scale production. This paper describes a novel injection-molded plastic array 3D culture (IMPACT) platform that incorporates a microfluidic design to integrate patterned 3D cell cultures within a single 96-well (diameter = 9 mm) plate. Cell containing gels can be sequentially patterned by capillary-guided flow along the corner and narrow gaps designed within the 96-well form factor. Compared to PDMS-based hydrophobic burst valve designs, this work utilizes hydrophilic liquid guides to obtain rapid and reproducible patterned gels for co-cultures. When a liquid droplet (i.e. cell containing fibrin or collagen gel) is placed on a corner, spontaneous patterning is achieved within 1 second. Optimal dimensionless parameters required for successful capillary loading have been determined. To demonstrate the utility of the platform for 3D co-culture, angiogenesis experiments were performed by patterning HUVEC (human umbilical endothelial cells) and LF (lung fibroblasts) embedded in 3D fibr...Continue Reading

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Citations

Jun 15, 2019·Advanced Healthcare Materials·Jihoon KoNoo Li Jeon
Jan 10, 2020·Lab on a Chip·Ulri N LeeErwin Berthier
May 12, 2020·American Journal of Physiology. Renal Physiology·Tianzi ZhangAshleigh B Theberge
Jun 13, 2020·Analytical Methods : Advancing Methods and Applications·Samuel B BerryAshleigh B Theberge
Jun 18, 2020·Frontiers in Bioengineering and Biotechnology·Jia-Wei YangGuan-Yu Chen
Dec 12, 2020·PloS One·Gabriel Santos RosalemMaria Gabriela Reis Carvalho
Dec 15, 2020·Biomaterials·Anna Fritschen, Andreas Blaeser
Jan 31, 2021·Science Advances·Sujin HyungNoo Li Jeon
Mar 26, 2021·Biotechnology and Bioengineering·Suryong KimNoo Li Jeon
Jun 23, 2021·Acta Biomaterialia·Seokyoung BangHong Nam Kim
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
Jul 2, 2019·Analytical Chemistry·Erwin BerthierAshleigh B Theberge
Jan 13, 2022·Neural Regeneration Research·Taiwei DongJian Wang

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Methods Mentioned

BETA
chip
Fluorescence
chips

Software Mentioned

IMPACT
EnvisionTec
Surface Evolver

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