PMID: 6169690Jan 1, 1981Paper

Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA-specific fluorochromes as probes of chromatin organization

Histochemistry
R R Cowden, S K Curtis

Abstract

The ability of the highly condensed chromatin of small thymocyte nuclei and the more loosely organized chromatin of hepatocyte nuclei to interact with nine DNA-specific fluorochromes was assessed by microfluorometry. Although the results obtained with five of the fluorochromes - mithramycin, 7-aminoactinomycin D, Hoechst 33258, DAPI, and propidium iodide - were found to be virtually unaffected by differences in the degree of condensation of the chromatin, the values obtained with the remaining fluorochromes - proflavine, quinacrine mustard, berberine sulfate, and pyronin Y - appeared to be affected significantly by organizational differences of the chromatin. All of the latter "structural probes," except quinacrine mustard, produced fluorescence values which were higher in the 2c nuclei of hepatocytes than in the nuclei of small thymocytes. Quinacrine mustard yielded higher values in thymocyte nuclei; and in the hepatocyte polyploid series (2, 4, and 8c), it did not produce the expected multiples of the 2c value. Pretreatment of the two types of nuclei with RNase affected their total fluorescence in unpredictable ways. While RNase extraction lessened the differences between thymocyte and 2c hepatocyte nuclei stained with propid...Continue Reading

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Citations

Jan 1, 1993·Cytometry·V J BirsteinB F Goncharov
Feb 7, 1998·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J Grosman, N J Vardaxis
Aug 1, 1994·Idengaku zasshi·M MurataF Motoyoshi
Oct 16, 1999·Cytometry·A E Vinogradov
Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Jan 1, 1991·Genetica·A M Fernández-PeraltaJ J González-Aguilera
Sep 15, 2000·Journal of Immunological Methods·I VermesC Reutelingsperger

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