Microglia P2Y13 Receptors Prevent Astrocyte Proliferation Mediated by P2Y1 Receptors

Frontiers in Pharmacology
Clara QuintasGlória Queiroz

Abstract

Cerebral inflammation is a common feature of several neurodegenerative diseases that requires a fine interplay between astrocytes and microglia to acquire appropriate phenotypes for an efficient response to neuronal damage. During brain inflammation, ATP is massively released into the extracellular medium and converted into ADP. Both nucleotides acting on P2 receptors, modulate astrogliosis through mechanisms involving microglia-astrocytes communication. In previous studies, primary cultures of astrocytes and co-cultures of astrocytes and microglia were used to investigate the influence of microglia on astroglial proliferation induced by ADPβS, a stable ADP analog. In astrocyte cultures, ADPβS increased cell proliferation through activation of P2Y1 and P2Y12 receptors, an effect abolished in co-cultures (of astrocytes with ∼12.5% microglia). The possibility that the loss of the ADPβS-mediated effect could have been caused by a microglia-induced degradation of ADPβS or by a preferential microglial localization of P2Y1 or P2Y12 receptors was excluded. Since ADPβS also activates P2Y13 receptors, the contribution of microglial P2Y13 receptors to prevent the proliferative effect of ADPβS in co-cultures was investigated. The results ...Continue Reading

References

Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Nov 1, 1994·British Journal of Pharmacology·R G HumphriesP Leff
Jun 20, 2003·Molecular Pharmacology·Frederic MarteauNathalie Suarez Gonzalez
Jun 9, 2004·Biochemical Pharmacology·Marta FumagalliMaria P Abbracchio
May 17, 2005·Nature Neuroscience·Dimitrios DavalosWen-Biao Gan
May 26, 2005·Biochemical Pharmacology·Yong-Chul KimKenneth A Jacobson
Aug 29, 2006·Biochemical Pharmacology·Liaman K MamedovaKenneth A Jacobson
Nov 23, 2006·Nature Neuroscience·Sharon E HaynesDavid Julius
Oct 17, 2007·Journal of Neuroinflammation·Josep Saura
Oct 30, 2007·Nature Neuroscience·Uwe-Karsten Hanisch, Helmut Kettenmann
Apr 12, 2008·Purinergic Signalling·Luz María G CarrasqueroM Teresa Miras-Portugal
Jan 13, 2009·Trends in Neurosciences·Francesco Di VirgilioMaria P Abbracchio
May 22, 2009·Journal of Neurochemistry·Luz María G CarrasqueroM Teresa Miras-Portugal
Aug 27, 2009·Journal of Neuroinflammation·Jessica M CrainJyoti J Watters
Sep 29, 2009·Cerebral Cortex·Susanna AmadioCinzia Volonté
Feb 12, 2010·Molecular Neurobiology·Dan ZhangJau-Shyong Hong
Aug 5, 2010·Journal of Neurochemistry·Roberta De SimoneLuisa Minghetti
Oct 21, 2010·Current Protocols in Toxicology·Mingwei Ni, Michael Aschner
May 12, 2011·Purinergic Signalling·Clara QuintasGlória Queiroz
Oct 19, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mary E HambyMichael V Sofroniew
Jul 3, 2013·Methods in Molecular Biology·Tomas Deierborg
Jan 28, 2014·Neuron·Joshua E Burda, Michael V Sofroniew
Nov 9, 2014·Cold Spring Harbor Perspectives in Biology·Michael V Sofroniew
Jan 19, 2017·Nature·Shane A LiddelowBen A Barres

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Citations

Apr 19, 2019·Journal of Leukocyte Biology·Aline L PachecoKarina R Bortoluci
Nov 20, 2018·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Colm CunninghamAna Belen Lopez-Rodriguez
Jul 29, 2020·International Journal of Molecular Sciences·Marcin Szustak, Edyta Gendaszewska-Darmach
Jul 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Armelle T MbavengThomas Efferth
Jun 17, 2020·Molecular Imaging·Hamideh Zarrinmayeh, Paul R Territo
Oct 18, 2020·International Journal of Molecular Sciences·Ciro De LucaAnna Maria Colangelo
Nov 28, 2020·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·E F BarinovT I Faber
Sep 18, 2020·Neuroscience·Rashmi Das, Subashchandrabose Chinnathambi
Jan 14, 2021·International Journal of Molecular Sciences·Juan Carlos Gil-RedondoEsmerilda G Delicado
Apr 4, 2021·International Journal of Molecular Sciences·Chrisanne Dsouza, Svetlana V Komarova
Apr 13, 2021·Frontiers in Cellular Neuroscience·Thomas Ernest James Phillips, Emily Maguire
Aug 3, 2021·Purinergic Signalling·Yuting ZouShangdong Liang

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Methods Mentioned

BETA
scintillation spectrometry
PCR
PCRs
electrophoresis
ELISA

Software Mentioned

CFX Manager
Beacon Designer TM
BLAST

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