PMID: 9630473Jun 19, 1998Paper

Microglia-specific localisation of a novel calcium binding protein, Iba1

Brain Research. Molecular Brain Research
D ItoS Kohsaka

Abstract

Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells [Imai et al. Biophys. Biochem. Res. Commun., 224 (1996) 855-862]. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain, respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo, and that Iba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.

References

Jan 1, 1992·Brain Research. Molecular Brain Research·S SaitohS Kohsaka
Jan 1, 1992·Brain Research. Brain Research Reviews·W E Thomas
Nov 15, 1991·The Journal of Comparative Neurology·J Gehrmann, G W Kreutzberg
Oct 1, 1991·Journal of Neurochemistry·N TakeiS Kohsaka
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J Streit
Oct 1, 1987·The Journal of Experimental Medicine·V H Perry, S Gordon
Jun 1, 1988·Trends in Neurosciences·V H Perry, S Gordon
Nov 1, 1989·Trends in Neurosciences·A PersechiniR H Kretsinger
Jan 1, 1989·Journal of Neuroscience Research·M B GraeberG W Kreutzberg
Jan 1, 1988·Glia·W J StreitG W Kreutzberg
Feb 8, 1988·The Journal of Comparative Neurology·W J Streit, G W Kreutzberg
Mar 10, 1988·Neuroscience Letters·M B GraeberG W Kreutzberg
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Jan 1, 1987·Acta Neuropathologica·A ProbstJ Ulrich

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Citations

Aug 14, 2001·Biochemical and Biophysical Research Communications·Y SasakiY Imai
May 16, 2002·Experimental Neurology·Silvia Rathke-HartliebJörg W Bartsch
Dec 17, 2009·Acta Neuropathologica·Manuel B Graeber, Wolfgang J Streit
Aug 19, 2011·Acta Neuropathologica·Qing-Shan Xue, Wolfgang J Streit
Sep 27, 2005·Histochemistry and Cell Biology·Nobuhiko OhnoShinichi Ohno
Apr 26, 2012·Brain Structure & Function·Zhi-Gang YaoChuan Qin
Nov 13, 2012·Cell and Tissue Research·Colleen L ZhuNorberto M Grzywacz
Jun 15, 2006·Cellular and Molecular Neurobiology·Kamila SaganováIvo Vanický
Jul 4, 2012·Clinical & Experimental Metastasis·Anna Sophie BerghoffRomana Höftberger
Jun 23, 2012·Journal of Molecular Neuroscience : MN·Kobra NaseriMasoumeh Jorjani
Jul 25, 2009·Molecular Neurobiology·Marina A Lynch
Aug 13, 2009·Journal of Ocular Biology, Diseases, and Informatics·Kin ChiuKwok-Fai So
Jan 5, 2010·Journal of Ocular Biology, Diseases, and Informatics·Kin ChiuKwok-Fai So
Dec 12, 2003·Brain Research. Molecular Brain Research·Isamu MoriYukihiro Nishiyama
Apr 14, 2000·Brain Research. Molecular Brain Research·F López-RedondoS Kohsaka
Jul 3, 2003·Molecular and Cellular Neurosciences·Mathias MäurerReinhard Kiefer
May 30, 2001·Microbes and Infection·I Mori, Y Kimura
Feb 28, 2002·The European Journal of Neuroscience·I BechmannR Nitsch
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Weiguo PengMaiken Nedergaard
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sophia T PapadeasNicholas J Maragakis
May 18, 2005·The Journal of Experimental Medicine·Mei MassengaleIrving L Weissman
Sep 17, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Rebecca A Sosa, Thomas G Forsthuber
Aug 2, 2008·Brain : a Journal of Neurology·Ansi ChangBruce D Trapp
Mar 4, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yuko TanakaYoichi Nakazato
Jul 1, 2008·Neuromodulation : Journal of the International Neuromodulation Society·Katsunori ShijoTakamitsu Yamamoto

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