PMID: 9009156Jan 17, 1997Paper

Microglial cells induce cytotoxic effects toward colon carcinoma cells: measurement of tumor cytotoxicity with a gamma-glutamyl transpeptidase assay

International Journal of Cancer. Journal International Du Cancer
J MurataL Juillerat-Jeanneret

Abstract

Activated macrophages have been shown to exert cytostatic and cytotoxic effects toward tumor cells via nitric oxide (NO) release. In the CNS, microglial cells are considered to be the main resident population of immune effector cells. In this study, cytotoxic activity of N11, an immortalized murine microglial cell line, toward rat progressive DHD/PROb and regressive DHD/REGb colon carcinoma cells was examined in parallel with NO production. Cytotoxicity was evaluated using a novel method, the gamma-glutamyl transpeptidase (gamma-GTP) assay, based on the fact that DHD tumor cells expressed high levels of gamma-GTP activity, while no gamma-GTP activity was found in cells of the monocyte/macrophage lineage. Results showed that activation of N11 cells by interferon-gamma plus either lipopolysaccharide or tumor necrosis factor-alpha induced high amounts of NO release and cytotoxic effects toward DHD/PROb as well as DHD/REGb cells. NO release by activated N11 cells was augmented by addition of tumor cell-conditioned medium. Both NO release by N11 cells and cytotoxicity were blocked by addition of N(G)-monomethyl-L-arginine (L-NMA), an inhibitor of NO synthase, suggesting that cytotoxicity was mediated by N11-derived NO. However, in t...Continue Reading

Citations

Dec 15, 2010·Translational Oncology·Emily C BrantleyIsaiah J Fidler
Jun 15, 2013·Oxidative Medicine and Cellular Longevity·Nicola TraversoCinzia Domenicotti
Apr 14, 2006·Journal of Neuro-oncology·Christopher L MarianiWolfgang J Streit
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qing ZhouWilliam J Murphy
Feb 28, 2013·International Journal of Molecular Sciences·Yi-Wen ZangZong-You Chen
Jan 1, 2012·Cancers·Mihaela Lorger
Jul 6, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·L Juillerat-JeanneretF Pinet
Apr 13, 2006·The Journal of Pharmacology and Experimental Therapeutics·Feride CengelliLucienne Juillerat-Jeanneret
Jan 3, 2012·Indian Journal of Clinical Biochemistry : IJCB·Safia Habib, Asif Ali
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Apr 30, 2013·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·W-z HeL-p Xia
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Sep 15, 2006·Neurochemistry International·Dora Lam-HimlinRudy J Castellani

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