Microglial response to transient focal cerebral ischemia: an immunocytochemical study on the rat cerebral cortex using anti-phosphotyrosine antibody

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
K KorematsuY Ushio

Abstract

Microglial response to transient focal ischemia was examined using an immunohistochemical method with a monoclonal antibody to phosphotyrosine (P-Tyr). For this purpose, a rat model of reversible middle cerebral artery occlusion for 1 h was used. Compared with results in the noninsulted hemisphere, there was a significant increase in P-Tyr immunolabeling of the microglia in the insulted cerebral cortex 3 h postreperfusion. This microglial reaction progressed up to 24 h after ischemic insult. In the affected cerebral cortex, morphological changes of the microglial positive for P-Tyr were also observed, with shortened and thickened processes, enlarged cell bodies, and ameboid features. Cell density analysis did not show any apparent change in number of P-Tyr-positive microglia in the insulted cortex at 6, 12, and 24 h after reperfusion, suggesting that the cells with increased P-Tyr immunoreactivity were resident microglia. The present findings suggest that signal transduction mediated by tyrosine phosphorylation is involved in the microglial response to ischemic injury in the rat cerebral cortex.

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Citations

Nov 20, 1998·Journal of Neuroscience Research·V R LombardiR Cacabelos
Apr 28, 2010·Bioconjugate Chemistry·Marytheresa A IfedibaAnna Moore
Feb 20, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·O PetersU Dirnagl
Jun 23, 2010·Chinese Medicine·Tsai-Hui Lin, Ching-Liang Hsieh
Sep 30, 2010·Chinese Medicine·Hsiang-Ni Chen, Ching-Liang Hsieh
Feb 7, 2016·Progress in Neurobiology·Yuanyuan MaGuo-Yuan Yang
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Jan 1, 1996·Advances in Neuroimmunology·J Zielasek, H P Hartung
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Apr 4, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daisuke OkutaniMingyao Liu
Mar 11, 2020·CNS Neuroscience & Therapeutics·Ruo-Xue WenZhi-Jun Zhang

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