Microgramma vacciniifolia (Polypodiaceae) fronds contain a multifunctional lectin with immunomodulatory properties on human cells

International Journal of Biological Macromolecules
Leydianne Leite de Siqueira PatriotaThiago Henrique Napoleão

Abstract

In this study, we report the purification and characterization of a multifunctional lectin (MvFL) from Microgramma vacciniifolia fronds as well as its immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). MvFL (pI 4.51; 54kDa) is a glycoprotein able to inhibit trypsin activity and that has sequence similarities (32% coverage) with a plant RNA-binding protein. Hemagglutinating activity of MvFL was not altered by heating at 100°C for 30min, but was reduced in alkaline pH (8.0 and 9.0). Fluorimetric analyses showed that this lectin did not undergo marked conformational changes when heated. However, the MvFL conformation changed depending on the pH. MvFL at 6.25-25μg/mL was not cytotoxic to lymphocytes present among PBMCs. The PBMCs incubated for 24h with the lectin (12.5μg/mL) showed increased TNF-α, IFN-γ, IL-6, IL-10, and nitric oxide production. MvFL also stimulated T lymphocytes from PBMCs to differentiate into CD8(+) cells. The activation (indicated by CD28 expression) of these cells was also stimulated. In conclusion, MvFL is a heat-stable and multifunctional protein, with both lectin and trypsin inhibitor activities, and capable of inducing predominantly a Th1 response in human PBMCs as well as ac...Continue Reading

Citations

Mar 18, 2020·3 Biotech·Ardilles Juan Carlos Alves Dos SantosEmmanuel Viana Pontual
Aug 18, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Thamara Figueiredo ProcópioThiago Henrique Napoleão
Apr 25, 2021·Chemistry & Biodiversity·Gabriela Cavalcante da SilvaThiago Henrique Napoleão
Mar 2, 2019·International Journal of Biological Macromolecules·Yufeng WangBaohua Li
Nov 17, 2020·Protein and Peptide Letters·Leydianne Leite de Siqueira PatriotaThiago Henrique Napoleáo
Jan 19, 2019·Journal of Ethnopharmacology·Dalila de Brito Marques RamosThiago Henrique Napoleão

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