Microgravity-Induced Transcriptome Adaptation in Mouse Paraspinal longissimus dorsi Muscle Highlights Insulin Resistance-Linked Genes

Frontiers in Physiology
Guido GambaraDieter Blottner

Abstract

Microgravity as well as chronic muscle disuse are two causes of low back pain originated at least in part from paraspinal muscle deconditioning. At present no study investigated the complexity of the molecular changes in human or mouse paraspinal muscles exposed to microgravity. The aim of this study was to evaluate longissimus dorsi adaptation to microgravity at both morphological and global gene expression level. C57BL/N6 male mice were flown aboard the BION-M1 biosatellite for 30 days (BF) or housed in a replicate flight habitat on ground (BG). Myofiber cross sectional area and myosin heavy chain subtype patterns were respectively not or slightly altered in longissimus dorsi of BF mice. Global gene expression analysis identified 89 transcripts differentially regulated in longissimus dorsi of BF vs. BG mice. Microgravity-induced gene expression changes of lipocalin 2 (Lcn2), sestrin 1(Sesn1), phosphatidylinositol 3-kinase, regulatory subunit polypeptide 1 (p85 alpha) (Pik3r1), v-maf musculoaponeurotic fibrosarcoma oncogene family protein B (Mafb), protein kinase C delta (Prkcd), Muscle Atrophy F-box (MAFbx/Atrogin-1/Fbxo32), and Muscle RING Finger 1 (MuRF-1) were further validated by real time qPCR analysis. In conclusion, ou...Continue Reading

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Citations

Apr 14, 2018·Radiation Research·Afshin BeheshtiSylvain V Costes
Apr 5, 2018·Frontiers in Physiology·Yunfang GaoNandu Goswami
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Aug 24, 2021·Frontiers in Cell and Developmental Biology·Yin LiangKeiji Naruse

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Datasets Mentioned

BETA
GSE94381

Methods Mentioned

BETA
in vitro transcription
PCR
biopsy

Software Mentioned

iQ5
BLAST
DAVID
Meta Series
ImageJ
Primer3
Leica
LAS X SP8
Affymetrix
GraphPad

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