Microheterogeneity in 16S ribosomal DNA-defined bacterial populations from a stratified planktonic environment is related to temporal changes and to ecological adaptations.

Applied and Environmental Microbiology
E O CasamayorRudolf Amann

Abstract

Temporal changes of the bacterioplankton from a meromictic lake (Lake Vilar, Banyoles, Spain) were analyzed with four culture-independent techniques: epifluorescence microscopy, PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting, fluorescence in situ whole-cell hybridization and flow cytometry sorting. Microscopically, blooms of one cyanobacterium (Synechococcus sp.-like), one green sulfur bacterium (Chlorobium phaeobacteroides-like), and one purple sulfur bacterium (Thiocystis minor-like) were observed at different depths and times. DGGE retrieved these populations and, additionally, populations related to the Cytophaga-Flavobacterium-Bacteroides phylum as predominant community members. The analyses of partial 16S ribosomal DNA sequences from the DGGE fingerprints (550 bp analyzed) revealed higher genetic diversity than expected from microscopic observation for most of these groups. Thus, the sequences of two Synechococcus spp. (both had a similarity of 97% to Synechococcus sp. strain PCC6307 in 16S rRNA), two Thiocystis spp. (similarities to Thiocystis minor of 93 and 94%, respectively), and three Cytophaga spp. (similarities to Cytophaga fermentans of 88 and 89% and to Cytophaga sp. of 93%, respectively) were ...Continue Reading

References

Jan 11, 1992·International Journal of Systematic Bacteriology·G E FoxP Jurtshuk
Mar 12, 1992·Nature·J A FuhrmanA A Davis
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E F DeLong
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 3, 1990·Nature·S J GiovannoniK G Field
Jun 1, 1987·Microbiological Reviews·C R Woese
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M BarnsN R Pace
Feb 1, 1996·Applied and Environmental Microbiology·M T Suzuki, S J Giovannoni
Jun 1, 1996·Journal of Bacteriology·R AmannK H Schleifer
Jun 1, 1996·Applied and Environmental Microbiology·J BornemanE W Triplett
Jan 1, 1997·Applied and Environmental Microbiology·K G FieldS J Giovannoni
Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Oct 23, 1997·International Journal of Systematic Bacteriology·T PalysF M Cohan
Nov 15, 1997·Applied and Environmental Microbiology·G WallnerR Amann
Feb 6, 1998·FEMS Microbiology Reviews·F von WintzingerodeE Stackebrandt
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W B WhitmanW J Wiebe
Mar 6, 1999·Current Opinion in Microbiology·D M Ward
May 2, 2000·Applied and Environmental Microbiology·R MassanaC Pedrós-Alió
Mar 1, 1993·Trends in Ecology & Evolution·C Pedrós-Alió

❮ Previous
Next ❯

Citations

Mar 16, 2007·Archives of Microbiology·Laura VillanuevaRicardo Guerrero
Dec 18, 2007·Microbial Ecology·Martínez-Alonso MairaNuria Gaju
Nov 26, 2010·Microbial Ecology·Albert Barberán, Emilio O Casamayor
May 25, 2012·Microbial Ecology·Mireia BartronsEmilio O Casamayor
Sep 14, 2010·Extremophiles : Life Under Extreme Conditions·Raja SayehDaniel Prieur
Oct 30, 2009·Journal of Environmental Sciences (China)·Xiao TanMin Zhang
Jun 20, 2002·Environmental Microbiology·Susana BenllochFrancisco Rodríguez-Valera
Jul 16, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark L Blaxter
Jan 15, 2008·Applied and Environmental Microbiology·Jennifer W EdmondsMary Ann Moran
Dec 25, 2012·Applied and Environmental Microbiology·Virginia LozaPilar Mateo
Feb 7, 2003·Applied and Environmental Microbiology·Shaheen B HumayounJames T Hollibaugh
Nov 5, 2005·Applied and Environmental Microbiology·Juan M Medina-SánchezEmilio O Casamayor
Apr 20, 2004·Journal of Bacteriology·Silvia G AcinasMartin F Polz
Mar 1, 2012·PLoS Biology·Hinsby Cadillo-QuirozRachel J Whitaker
Jan 1, 2014·Bioresource Technology·Shuzhen LiGuohua Gu
Nov 6, 2013·Systematic and Applied Microbiology·Cristina Díez-VivesSilvia G Acinas
May 31, 2013·Mikrobiologiia·L A Magdanova, N V Goliasnaia
Jun 10, 2004·Environmental Microbiology·Vanja Klepac-CerajMartin F Polz
Apr 12, 2005·Environmental Microbiology·Kaisa HaukkaKaarina Sivonen
Jan 25, 2008·Environmental Microbiology·Jean-Christophe Auguet, Emilio O Casamayor
Mar 15, 2011·Molecular Ecology·Albert BarberánEmilio O Casamayor
Sep 1, 2015·Systematic Biology·David J Krause, Rachel J Whitaker
May 9, 2006·Trends in Microbiology·Carlos Pedrós-Alió
Aug 12, 2010·FEMS Microbiology Ecology·Zhiguo HeGuanzhou Qiu
Sep 11, 2007·FEMS Microbiology Ecology·Sven BeckerAnneliese Ernst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.