PMID: 6172375Dec 1, 1981Paper

Microheterogeneity in regulatory circuits for T-cell alloresponsiveness

Immunology
R M Gorczynski, S MacRae

Abstract

Mixed leucocyte cultures of responder spleen cells from individual B10 mice, challenged with irradiated allogeneic B10.D2 or B10.BR spleen cells, were used to generate discrete pools of cytotoxic T lymphocytes (CTL) with which to immunize (B10 x B10.D2)F1 or (B10 x B10.BR)F1 animals. Aliquots of the original donor B10 spleen cells were stored at -70 degrees. Eighteen days after immunization of the F1 animals, spleen and serum preparations from these mice were tested, in reciprocal fashion, for their ability to affect the development of CTL from the thawed donor B10 spleen lymphocytes in fresh cultures challenged with either the original or a third party allogeneic stimulus. Evidence for individual specific suppression by F1-T lymphocytes or by F1-serum (antibody) molecules was obtained. By priming (B10 x B10.D2)F1 mice with B10 lymphoid cells the F1 animals can also be shown to resist the otherwise lethal GvHD induced by sublethal whole body irradiation followed by intravenous challenge with B10 lymphoid cells. Using F1 mice primed and subsequently challenged with lymphocytes prepared from individual donors, self-preference in the regulation of GvHD was also seen. A similar fine specificity of regulatory cells was observed usin...Continue Reading

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