PMID: 6171874Jul 1, 1981Paper

Microheterogeneity of alpha-fetoprotein in patient serum as demonstrated by lectin affino-electrophoresis

Scandinavian Journal of Immunology
J BreborowiczD Breborowicz

Abstract

Microheterogeneity of alpha-fetoprotein (AFP) present in the sera of 76 patients was studied by lectin affino-immunoelectrophoresis. Seventeen patients had benign liver disorders and the remaining 59 patients were treated for primary or secondary liver cancer or yolk sac tumour. By means of Con A, AFP was divided into two variants, while lentil lectin (LCA) made it possible to separate AFP in three variants. In some patients the relative concentrations of Con A and LCA AFP variants were similar; these patients were believed to produce AFP of the same 'profile'. Fourteen AFP profiles were observed by estimation of the area enclosed by precipitates corresponding to respective AFP variants. It was also possible to estimate the AFP profile on the basis of a simple visual analysis of the electrophoretic plates. The obtained results indicate that the AFP profiles of patients with cancer were variable. In spite of variations of the AFP profile in cancer patients, in most cases it was possible to differentiate primary liver cancer from yolk sac tumour and from liver metastases of cancer. In addition, in two-thirds of hepatoma patients the AFP profile was different from the profile observed in patients with benign liver disorders.

References

Nov 15, 1978·International Journal of Cancer. Journal International Du Cancer·E RuoslahtiM Seppälä
Aug 29, 1970·Lancet·L R PurvesI Bersohn
Oct 30, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·B Norgaard-Pedersen

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Citations

Jan 1, 1990·Journal of Clinical Laboratory Analysis·T KohnoE Ishikawa
Feb 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·N KinoshitaN Taniguchi
Jun 30, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A Turner
Jan 31, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ShimizuK Taketa
Oct 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ShimizuS Matsuura
Aug 24, 1987·Journal of Immunological Methods·J BreborowiczJ Klosin
Dec 2, 1999·Biochimica Et Biophysica Acta·E MiyoshiN Taniguchi
Jun 24, 1993·The New England Journal of Medicine·Y SatoS Nagataki
Jan 26, 2008·Journal of Biochemistry·Eiji MiyoshiTsutomu Nakagawa
Aug 26, 1998·Journal of Gastroenterology and Hepatology·S MoriH Asakura
Mar 23, 2011·Journal of Gastroenterology and Hepatology·Yutaka AoyagiTakeshi Suda
Jul 24, 2010·Journal of Oncology·Eiji MiyoshiYoko Miyoshi
May 1, 1989·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·K OhkawaY Terashima
Mar 25, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Tomomi Masuda, Eiji Miyoshi
Jan 27, 2010·World Journal of Gastroenterology : WJG·Piotr StefaniukAlicja Wiercinska-Drapalo
Jan 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Timothy M BlockAnand S Mehta
Mar 27, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E HansenT C Bøg-Hansen
Nov 14, 2015·Journal of Proteomics·Meritxell BalmañaRosa Peracaula
Jun 10, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Evi N Debruyne, Joris R Delanghe
Mar 26, 2016·Frontiers in Oncology·Stefan MereiterCelso A Reis
Oct 11, 2008·Mass Spectrometry Reviews·Rita BaroneDomenico Garozzo
Apr 25, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yu SaitoMichihito Asanoma
Sep 1, 1984·Placenta·A MackiewiczJ Breborowicz
Oct 7, 2006·The Journal of Surgical Research·Iain H McKillopLeonidas G Koniaris
Jan 1, 1983·Annals of the New York Academy of Sciences·C J SmithP C Kelleher
Jan 1, 1983·Annals of the New York Academy of Sciences·K TaketaE Ichikawa
Jan 1, 1996·Clinical Oncology : a Journal of the Royal College of Radiologists·P G de TakatsM H Cullen
Mar 13, 2014·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Satoshi YamagiwaYutaka Aoyagi

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