PMID: 6168384Jul 1, 1981Paper

Microinjection of anticlathrin antibodies into fibroblasts does not interfere with the receptor-mediated endocytosis of alpha2-macroglobulin

Cell
J WehlandI Pastan

Abstract

Affinity-purified antibodies prepared against the major coat protein of brain coated vesicles, clathrin, were microinjected into cultured fibroblasts, and their intracellular distribution was followed by immunofluorescence microscopy and ultrastructural immunocytochemistry. Microinjected anticlathrin antibodies were concentrated on coated regions of the plasma membrane and the GERL apparatus. When an excess of anticlathrin antibodies was injected into the cytosol, coated pits on the plasma membrane were covered by anticlathrin antibody but still functioned to cluster an internalize alpha2-macroglobulin. These results are discussed in terms of the role of clathrin in the pathway of receptor-mediated endocytosis. Our data indicate that in cultured fibroblasts coated pits are stable elements permanently attached to the plasma membrane.

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Citations

Jun 1, 1990·The Journal of Membrane Biology·G S Payne
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C Willingham, I Pastan
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W GodfreyL Wofsy
Jul 1, 1982·The Journal of Cell Biology·M C Willingham, I H Pastan
Oct 11, 2003·Molecular Biology of the Cell·Howard S MoskowitzTimothy A Ryan
Jul 31, 1987·Cell·S J DoxseyA Helenius
Aug 7, 1983·Journal of Theoretical Biology·D N McKinley
May 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C J Steer, R D Klausner
Jan 1, 1982·Annals of the New York Academy of Sciences·R B DicksonI H Pastan
Jun 1, 1988·Molecular and Cellular Biology·J R Carlson
Aug 1, 1987·Experimental Cell Research·M C WillinghamA V Rutherford
Jun 1, 1986·Experimental Cell Research·G Bruder, B Wiedenmann

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