PMID: 9421533Feb 28, 1998Paper

Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes

Nucleic Acids Research
T de WitF Grosveld

Abstract

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P C OrbanJ D Marth
Jul 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·M LaksoH Westphal
Dec 1, 1990·Trends in Biochemical Sciences·R J JacksonA Kaminski
Sep 1, 1990·Molecular and Cellular Biology·Y ZhouW T McAllister
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Sauer, N Henderson
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K ArakiP Vassalli
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·P MountfordA Smith
May 11, 1993·Nucleic Acids Research·W Baubonis, B Sauer
Mar 15, 1997·Nucleic Acids Research·J EllisF Grosveld

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Citations

Oct 26, 2010·Plant Cell Reports·Yueju WangJames G Thomson
Oct 6, 1999·Current Opinion in Biotechnology·D Metzger, R Feil
Apr 19, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andrew WilberXin Wang
May 15, 2002·Cloning and Stem Cells·Andreas F Kolb
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Roger P HollisMichele P Calos
Jan 7, 2000·Biochemical and Biophysical Research Communications·T P DaltonD W Nebert
Aug 4, 2009·Biochemical and Biophysical Research Communications·Kyoungmi KimDaekee Lee
Jul 26, 2011·Genesis : the Journal of Genetics and Development·Hui LiuPentao Liu
Feb 1, 2015·Cellular and Molecular Life Sciences : CMLS·Pablo BoschWilfried A Kues
Jun 11, 2011·Biochemical and Biophysical Research Communications·Bin WangDaniel W Nebert
Nov 18, 2000·Annals of the New York Academy of Sciences·D W NebertM J Carvan
Jul 28, 2020·Methods : a Companion to Methods in Enzymology·Irena JenickovaRadislav Sedlacek

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