Microinjection of cultured rat embryos: a new technique for studies in chemical dysmorphogenesis

Toxicology and Applied Pharmacology
K L Stark, M R Juchau

Abstract

The utility of a new technique for exposure of cultured whole rat embryos to potential dysmorphogens was demonstrated with nitrosofluorene (NF), a cytotoxic and mutagenic metabolite of 2-acetylaminofluorene (AAF). At an initial concentration in the culture medium of 41 microM, NF produced a 100% incidence of defects in axial rotation with no significant effect on prosencephalic development, consistent with previous reports. This route of exposure was also associated with a significant decrease in yolk sac vasculature and protein content. However, when 2 to 20 ng of NF was microinjected directly into the amniotic space, the predominant malformation observed was prosencephalic hypoplasia. Injection of 10 ng NF resulted in approximately equivalent decreases in viability as 41 microM NF dissolved in the culture medium, but produced only a 41% incidence of rotation defects and a 27% incidence of open neural tubes in the rhombencephalic region. The protein content of injected conceptuses was significantly reduced in the embryo, but not in the visceral yolk sac. When 10 ng of NF was injected inside the yolk sac but outside the amnion, the incidence of abnormal rotation was increased to 75%, and the severity of prosencephalic hypoplasi...Continue Reading

References

Feb 1, 1978·Biological Reviews of the Cambridge Philosophical Society·D A New
Jul 2, 1979·Life Sciences·A G FantelT H Shepard
Dec 1, 1976·Teratology·K E WilliamsJ B Lloyd
Jan 1, 1989·Annual Review of Pharmacology and Toxicology·M R Juchau
Oct 1, 1984·Toxicology and Applied Pharmacology·E M Faustman-WattsM R Juchau
May 15, 1982·The Biochemical Journal·T KooistraJ B Lloyd

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Citations

Nov 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Klug, D Neubert
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P F CumberlandM K Pratten
Jan 1, 1991·Reproductive Toxicology·Q P LeeM R Juchau
Jan 1, 1991·Reproductive Toxicology·M R JuchauA G Fantel
Aug 17, 1993·Biochemical Pharmacology·J C Kraft, M R Juchau

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