Microinjection of in vitro transcribed RNA and antisense oligonucleotides in mouse oocytes and early embryos to study the gain- and loss-of-function of genes

Molecular Biotechnology
I Kola, S H Sumarsono

Abstract

Mouse oocytes have proven useful in experiments aimed at studying gene function. They have been used to analyze the gain-of-function acquired after microinjection of RNA transcribed in vitro from specific gene constructs, and for establishing loss-of-function mutation obtained by injecting in vitro transcribed antisense RNA and/or synthetic oligonucleotides. This article presents protocols utilized in these studies. Specifically, the acquisition of mouse oocytes and/or embryos, the genesis of the necessary DNA and/or RNA to be used, and procedures for microinjection.

References

Jan 1, 1991·Molecular Reproduction and Development·J Hendrey, I Kola
Sep 1, 1985·The Journal of Cell Biology·R Harland, H Weintraub
Dec 1, 1985·Cell·C BirchmeierM Wigler
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W M Wormington
Feb 1, 1988·Molecular and Cellular Biology·M SchmidtG F Vande Woude
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T HoltA W Nienhuis
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D A Melton
Jun 6, 1985·Nature·F Propst, G F Vande Woude
Sep 1, 1982·Journal of Reproduction and Fertility·P QuinnD G Whittingham
Jan 23, 1981·Science·R L BrinsterM E Trumbauer
Jan 31, 1980·Nature·R L BrinsterM R Avarbock

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Citations

Aug 2, 2011·Journal of the American Society of Nephrology : JASN·Rachel K MillerPierre D McCrea
Dec 19, 2017·Zygote : the Biology of Gametes and Early Embryos·Maria do Carmo Faria Paes, Laura Satiko Okada Nakaghi

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