Microinjection of lectins, hyaluronidase, and hyaluronate fragments interferes with cleavage delay and mesoderm induction in embryos of Patella vulgata

Developmental Biology
W M Kühtreiber, C A van Dongen

Abstract

In embryos of Patella vulgata at the 32-cell stage, one of the four vegetally located macromeres makes contacts with overlying animal micromeres. As a result, this macromere (designated 3D) divides significantly later than the other macromeres and forms the mesodermal stem cell 4d. Shortly before and during this interaction two types of extracellular matrix are present: a basal lamina-like layer on the tips of the micromeres and a loose fibrillar meshwork in the blastocoel. In this paper we examine the role of the matrix in cleavage delay and mesoderm determination. The microinjection of extracellular matrix-binding lectins, or of hyaluronidase, or of decasaccharide fragments of hyaluronate into the blastocoel results in embryos in which either no or two macromeres are delayed in cleavage and are presumably determined as mesodermal stem cells. We suggest that the fibrillar meshwork is needed for macromere elongation toward the micromeres and that the basal lamina-like layer is involved in the determination process itself.

References

Jan 1, 1978·Physiological Reviews·W D Comper, T C Laurent
Aug 1, 1979·The Journal of Cell Biology·C B Underhill, B P Toole
Mar 1, 1986·Cell Differentiation·N VasanO La Manna
Jan 1, 1987·Differentiation; Research in Biological Diversity·W M Kühtreiber, F Serras
Sep 1, 1971·Developmental Biology·B P Toole, R L Trelstad
Jun 1, 1968·Biopolymers·D A GibbsE A Balazs
Jan 1, 1982·Connective Tissue Research·B P Toole
Nov 1, 1981·Cancer·T HoraiS Hattori
Oct 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·D Hamerman, D D Wood
Sep 1, 1984·Cell Biology International Reports·C D Stern
Feb 1, 1983·Cell Differentiation·E Belsky, B P Toole
Sep 17, 1981·Nature·A M RichS T Hoffstein
Jan 1, 1988·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Wiel M KühtreiberCees A M van Dongen
May 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Wiel M KühtreiberCees A M van Dongen

❮ Previous
Next ❯

Citations

Jun 15, 1992·Microscopy Research and Technique·M R Dohmen
Jan 1, 1994·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Peter Damen, Wim J A G Dictus
Nov 1, 1991·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Danica ZivkovicRené Dohmen
Apr 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Florenci Serras, Johanna E Speksnijder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.