PMID: 6402779Mar 1, 1983Paper

Microinjection of purified ornithine decarboxylase into Xenopus oocytes selectively stimulates ribosomal RNA synthesis

Proceedings of the National Academy of Sciences of the United States of America
D H Russell

Abstract

This study has utilized stage VI oocytes of Xenopus laevis which have amplified the rDNA gene 1,000-fold to assess whether the microinjection of ornithine decarboxylase (OrnDCase) would stimulate [alpha-32P]guanosine incorporation into 45S and 18S/28S RNA selectively. The injection of purified OrnDCase into individual oocytes resulted in a greater than 2-fold increase in the incorporation of [32P]guanosine into 45S RNA and 18S/28S RNA with no increased incorporation into low molecular weight RNA. Further, an irreversible inhibitor of OrnDCase, alpha-difluoromethylornithine (CHF2-Orn), rapidly inhibited the endogenous activity of OrnDCase when added to the buffered Hepes solution bathing the oocytes and also inhibited the incorporation of [32P]guanosine into rRNA. The inhibitory effect of CHF2-Orn could not be reversed totally by addition of 10 microM putrescine to the oocytes. OrnDCase injected into oocytes in the presence of CHF2-Orn in the media did not stimulate incorporation of [32P]guanosine label into rRNA. However, when CHF2-Orn was removed from the buffered medium at the time of the injection of label and enzyme, a 3-fold increase of 32P incorporation into 18S/28S RNA occurred. Therefore, in an in vivo model in which am...Continue Reading

References

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Citations

Aug 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·S M Whelly
Oct 5, 2007·Journal of Gastroenterology and Hepatology·Qianji NingQiang Shao
Oct 1, 1985·Muscle & Nerve·D Pette, G Vrbová
Jan 1, 1988·Experimental Cell Research·K TsurugiK Shiokawa
Sep 1, 1985·Experimental Neurology·W Tetzlaff, G W Kreutzberg
Mar 30, 1987·Biochemical and Biophysical Research Communications·G ScalabrinoA Bernelli-Zazzera
Dec 1, 1987·Biochemistry·M Caizergues-FerrerM O Olson
Feb 1, 1985·Journal of Neurochemistry·G A DienelS J Rosenfeld
Jan 1, 1984·Cell Biochemistry and Function·A M Kaye
Jan 1, 1984·Cell Biochemistry and Function·U Bachrach
May 1, 1988·The Journal of Experimental Zoology·S MiyataH K Kihara
Feb 1, 1995·Alcoholism, Clinical and Experimental Research·I A ShibleyS N Pennington
Dec 14, 2005·Molecular and Cellular Biochemistry·Kathryn LawsonSusan K Gilmour
Mar 1, 1987·Molecular and Cellular Biology·R H DavisP Eversole-Cire

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