Microirradiation techniques in radiobiological research

Journal of Biosciences
Guido A Drexler, Miguel J Ruiz-Gomez

Abstract

The aim of this work is to review the uses of laser microirradiation and ion microbeam techniques within the scope of radiobiological research. Laser microirradiation techniques can be used for many different purposes. In a specific condition, through the use of pulsed lasers, cell lysis can be produced for subsequent separation of different analytes. Microsurgery allows for the identification and isolation of tissue sections, single cells and subcellular components, using different types of lasers. The generation of different types of DNA damage, via this type of microirradiation, allows for the investigation of DNA dynamics. Ion microbeams are important tools in radiobiological research. There are only a limited number of facilities worldwide where radiobiological experiments can be performed. In the beginning, research was mostly focused on the bystander effect. Nowadays, with more sophisticated molecular and cellular biological techniques, ion microirradiation is used to unravel molecular processes in the field of radiobiology. These include DNA repair protein kinetics or chromatin modifications at the site of DNA damage. With the increasing relevance of charged particles in tumour therapy and new concepts on how to generat...Continue Reading

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Citations

Jan 9, 2018·International Journal of Radiation Biology·Mihaela GhitaKevin M Prise
Jul 19, 2019·Nucleic Acids Research·Jakub A KochanPrzemek M Krawczyk
Aug 3, 2019·Micromachines·Alfonso Blázquez-Castro
Sep 21, 2018·JCI Insight·Kandace GollompMortimer Poncz
Oct 20, 2020·Frontiers in Bioengineering and Biotechnology·Alfonso Blázquez-CastroJavier Santos

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