PMID: 8582976Jul 1, 1995Paper

Micromanipulation of mouse gametes with laser microbeam and optical tweezers

Human Reproduction
M E EnginsuS Gordts

Abstract

Micromanipulation of mouse gametes with a commercially available compact laser microbeam system was studied. Both the normal in-vitro fertilization (IVF) group and the laser zona dissection (LZD) group were tested under normal (2 x 10(6) motile spermatozoa/ml) and low (500,000 motile spermatozoa/ml) insemination conditions. Subzonal insemination (SUZI) was also tried in a small group of gametes and the results were compared with those of the low insemination groups. Fertilization rates and blastocyst formation rates for the IVF and the LZD-treated groups were respectively 53 and 60% and 60 and 78%, which were not significantly different. However, under low insemination conditions, the results were significantly better in the LZD-treated group (58% fertilization rate and 83% blastocyst formation rate) compared to the results of the IVF group (33 and 48%) (P < 0.05). The SUZI-treated group showed the lowest fertilization rate (18%). No significant difference between the LZD and the IVF group was observed with respect to parthenogenetic activation. LZD has a beneficial effect on fertilization rates in cases of reduced sperm quality.

Citations

Oct 1, 1996·Journal of Clinical Laser Medicine & Surgery
Jul 20, 2007·Proteomics·Ferdinand von EggelingGünther Ernst
Oct 14, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Dominika D PetersMartin Hrabé de Angelis
Sep 1, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Mo GuanMartin D Fray
May 4, 2012·Photomedicine and Laser Surgery·Tiina I Karu
Jun 25, 2020·Proteomics·Ferdinand von Eggeling, Franziska Hoffmann
Dec 23, 2016·Reports on Progress in Physics·Karl Otto Greulich

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