Micromarrows--three-dimensional coculture of hematopoietic stem cells and mesenchymal stromal cells

Tissue Engineering. Part C, Methods
Matthew M CookMichael R Doran

Abstract

Hematopoietic stem cell (HSC) transplant is a well established curative therapy for some hematological malignancies. However, achieving adequate supply of HSC from some donor tissues can limit both its application and ultimate efficacy. The theory that this limitation could be overcome by expanding the HSC population before transplantation has motivated numerous laboratories to develop ex vivo expansion processes. Pioneering work in this field utilized stromal cells as support cells in cocultures with HSC to mimic the HSC niche. We hypothesized that through translation of this classic coculture system to a three-dimensional (3D) structure we could better replicate the niche environment and in turn enhance HSC expansion. Herein we describe a novel high-throughput 3D coculture system where murine-derived HSC can be cocultured with mesenchymal stem/stromal cells (MSC) in 3D microaggregates--which we term "micromarrows." Micromarrows were formed using surface modified microwells and their ability to support HSC expansion was compared to classic two-dimensional (2D) cocultures. While both 2D and 3D systems provide only a modest total cell expansion in the minimally supplemented medium, the micromarrow system supported the expansion ...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Jan 1, 1997·British Medical Bulletin·S Riordan, R Williams
Jan 1, 2003·Cell·Tyler Jacks, Robert A Weinberg
May 9, 2006·Biomacromolecules·Tristan I CrollJustin J Cooper-White
Sep 14, 2006·Tissue Engineering·Yue ZhangKam W Leong
Apr 20, 2007·Annals of the New York Academy of Sciences·Anne WilsonAndreas Trumpp
Dec 19, 2007·Methods in Molecular Medicine·Nicholas E Timmins, Lars K Nielsen
Feb 13, 2010·Stem Cells and Development·Menno ter HuurneRuurd Torensma
Apr 10, 2010·Biomaterials·Michael R DoranJustin J Cooper-White
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Sep 8, 2010·European Cells & Materials·K JähnM J Stoddart

❮ Previous
Next ❯

Citations

Jun 1, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Karen BiebackUNKNOWN START-MSC consortium
Jul 3, 2013·Tissue Engineering. Part C, Methods·Bita CarrionAndrew J Putnam
Jan 23, 2014·Advanced Drug Delivery Reviews·Christoph Giese, Uwe Marx
Jul 12, 2014·Cell and Tissue Research·Christina L RettingerKai P Leung
Dec 3, 2014·Advanced Drug Delivery Reviews·J LeijtenF P Luyten
Mar 20, 2015·American Journal of Respiratory Cell and Molecular Biology·Khang M DuongSimon Phipps
Sep 12, 2015·Biotechnology Journal·Ji Sun ChoiBrendan A C Harley
Apr 30, 2015·Stem Cells and Development·Ann De Becker, Ivan Van Riet
Mar 20, 2016·Tissue Engineering. Part C, Methods·Yu-Suke TorisawaDonald Ingber
Mar 31, 2015·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Jeehye KimHojae Bae
Dec 23, 2016·Tissue Engineering. Part C, Methods·Mohammad HoushmandMahin Nikougoftar Zarif
Jan 1, 2013·Journal of Clinical Medicine·Matthew M CookKerry Atkinson
May 23, 2018·Nature Materials·Emily A GosselinChristopher M Jewell
Apr 29, 2014·World Journal of Stem Cells·Dario SiniscalcoNicola Antonucci
May 6, 2014·Nature Methods·Yu-suke TorisawaDonald E Ingber
Jun 29, 2017·Journal of Tissue Engineering and Regenerative Medicine·Stefan SieberMark Rosowski
Jun 5, 2019·Bioengineering·Cordula NiesEric Gottwald
Apr 6, 2019·Tissue Engineering. Part B, Reviews·Ganesh IngavleVaijayanti Kale
Apr 2, 2019·MRS Communications·Annamarija RaicCornelia Lee-Thedieck
Nov 16, 2017·Current Protocols in Stem Cell Biology·Haolu WangMichael S Roberts
May 8, 2018·Biochemical Engineering Journal·Xuegang YuanTeng Ma

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
flow cytometry
light microscopy
FACS

Software Mentioned

GraphPad Prism
ImageJ
GraphPad
FlowJo

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Related Papers

Biochemical and Biophysical Research Communications
Mahboubeh KabiriMichael R Doran
Journal of Bioscience and Bioengineering
Nicki PanoskaltsisJ H David Wu
Regenerative Medicine
Joan E NicholsJoaquin Cortiella
© 2021 Meta ULC. All rights reserved