Micromass cultures in teratology

Current Protocols in Toxicology
R A Ponce

Abstract

This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical, molecular, and immunophenotypic characteristics observed during in vivo differentiation. These cultures can be used in a wide variety of studies designed to investigate normal cellular ontogeny, the teratogenic potential of test agents, or the mechanisms underlying the cellular response to environmental stress.

References

Mar 1, 1992·Toxicology and Applied Pharmacology·S G Whittaker, E M Faustman
Jun 1, 1991·Toxicology and Applied Pharmacology·S G Whittaker, E M Faustman
May 1, 1988·Mutation Research·E M Faustman
Jan 1, 1986·Drug Metabolism Reviews·L P BrownG G Gibson
Jun 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L P BrownG G Gibson
Nov 1, 1984·Toxicology and Applied Pharmacology·O P Flint, T C Orton
Dec 1, 1982·Developmental Biology·M SolurshR S Reiter
Jun 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M R Seeley, E M Faustman
Jul 1, 1994·Toxicology and Applied Pharmacology·R A PonceE M Faustman

Related Concepts

Cell Differentiation Process
Embryonic Structures, Mammalian
Environmental Pollutants
Epithelial Cells
Midbrain Structure
Maternal Exposure
Limb Buds
Chondrocyte
Microdissection
Embryo Culture Techniques

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