Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages

Marine Drugs
Hao TangZhong-Fu Ma

Abstract

Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.

References

Jan 1, 1984·Methods in Enzymology·H Aebi
Jan 22, 2003·The British Journal of Dermatology·M FurueT Koga
Sep 24, 2004·Trends in Microbiology·Astrid M van der SarWilbert Bitter
May 5, 2007·BMC Developmental Biology·Chris HallPhil Crosier
Jul 8, 2008·Fish & Shellfish Immunology·P SwainS Dash
Jul 25, 2008·Nature·Alberto MantovaniFrances Balkwill
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
May 17, 2012·Biochemical Pharmacology·I-Ta Lee, Chuen-Mao Yang
Jul 9, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Saikat DewanjeeRanabir Sahu

❮ Previous
Next ❯

Citations

Apr 26, 2016·Biochemical and Biophysical Research Communications·Chunping LiChunting Wang
Aug 24, 2019·BMC Veterinary Research·Lu-Lu WangHong-Lin Ren
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Yufei XieMarcel J M Schaaf

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
transgenic
fluorescence microscopy
Assay

Software Mentioned

Prism
GraphPad
Image J

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.