Micromotor-Based Biosensing Using Directed Transport of Functionalized Beads

ACS Sensors
Sinwook Park, Gilad Yossifon

Abstract

Previous micromotor-based biosensing studies used to functionalize the surface of the micromotor with specific molecular probes for binding of target analyte, thus limiting the use of the micromotor for the specific target. In contrast, here, we introduce a novel approach of using a nonfunctionalized micromotor as a generic cargo carrier being able to perform label-free and dynamic loading, transport, and release of functionalized beads. Hence, such an approach enables one to use the same micromotor system for sensing of varying targets via different commercially available functionalized beads, demonstrating the use of micromotors as a practical and versatile means for biosensing. We have also introduced a simplified microfluidic design that can be used for immunosensing or DNA binding tests without necessity for complicated fluid handling (buffer exchange, washing, etc.) steps. We expect this approach to open up new realizations of simplified and generic biosensing platforms.

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Citations

Dec 12, 2020·Journal of Colloid and Interface Science·Sinwook ParkGilad Yossifon
Apr 1, 2021·Chemical Reviews·Huaijuan ZhouMartin Pumera
Mar 29, 2021·Biosensors & Bioelectronics·Qing GaoBaojun Li
Jul 31, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Jiaoyu RenHong Wang
Oct 29, 2021·Journal of Drug Targeting·Muhammad SuhailAshok Kumar Janakiraman
Nov 11, 2021·Mikrochimica Acta·Beatriz Jurado-SánchezAlberto Escarpa

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Methods Mentioned

BETA
biosensing
electrophoresis
Fluorescence
sandwich immunoassay

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