Micron-scale phase segregation in lipid monolayers induced by myelin basic protein in the presence of a cholesterol analog

Biochimica Et Biophysica Acta
Carla M RosettiN Wilke

Abstract

It was previously shown that myelin basic protein (MBP) can induce phase segregation in whole myelin monolayers and myelin lipid films, which leads to the accumulation of proteins into a separate phase, segregated from a cholesterol-enriched lipid phase. In this work we investigated some factors regulating the phase segregation induced by MBP using fluorescent microscopy of monolayers formed with binary and ternary lipid mixtures of dihydrocholesterol (a less-oxidable cholesterol analog) and phospholipids. The influence of the addition of salts to the subphase and of varying the lipid composition was analyzed. Our results show that MBP can induce a dihydrocholesterol-dependent segregation of phases that can be further regulated by the electrolyte concentration in the subphase and the composition (type and proportion) of non-sterol lipids. In this way, changes of the lipid composition of the film or the ionic strength in the aqueous media modify the local surface density of MBP and the properties (phase state and composition) of the protein environment.

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Citations

Oct 30, 2013·Cellular and Molecular Life Sciences : CMLS·Mostafa BakhtiMikael Simons
May 28, 2013·Current Opinion in Cell Biology·Mikael Simons, David A Lyons
Sep 18, 2012·Chemistry and Physics of Lipids·Andrea Alejandra Bischof, Natalia Wilke
Jan 21, 2012·Biophysical Journal·Larisa YurlovaMikael Simons
Jan 31, 2015·Soft Matter·Andrea Alejandra BischofNatalia Wilke
Dec 1, 2011·Biophysics Reviews·Natalia Wilke, Bruno Maggio
Nov 16, 2013·Plant Physiology·Patricia Gerbeau-PissotFrançoise Simon-Plas

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