Micron-scale positioning of features influences the rate of polymorphonuclear leukocyte migration

Biophysical Journal
J TanW M Saltzman

Abstract

Microfabrication technology was used to create regular arrays of micron-size holes (2 microm x 2 microm x 210 nm) on fused quartz and photosensitive polyimide surfaces. The patterned surfaces, which possessed a basic structural element of a three-dimensional (3-D) network (i.e., spatially separated mechanical edges), were used as a model system for studying the effect of substrate microgeometry on neutrophil migration. The edge-to-edge spacing between features was systematically varied from 6 microm to 14 microm with an increment of 2 microm. In addition, collagen was used to coat the patterned quartz surfaces in an attempt to change the adhesive properties of the surfaces. A radial flow detachment assay revealed that cell adhesion was the strongest on the quartz surface (approximately 50% cell attached), whereas it was relatively weaker on polyimide and collagen-coated quartz (approximately 25% cell attached). Cell adhesion to each substrate was not affected either by the presence of holes or by the spacing between holes. A direct visualization assay showed that neutrophil migration on each patterned surface could be characterized as a persistent random walk; the dependence of the random motility coefficient (mu) as a function...Continue Reading

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Citations

Jul 30, 2011·Annals of Biomedical Engineering·Y SakamotoM H Zaman
Dec 13, 2006·Biomedical Microdevices·Divya D NalayandaDavid W Schmidtke
Mar 17, 2004·Biomaterials·Jian Tan, W Mark Saltzman
May 19, 2004·Biomaterials·Catherine C BerryAdam S G Curtis
Sep 29, 2011·PloS One·Jennifer A Mitchel, Diane Hoffman-Kim
May 14, 2004·Critical Reviews in Biomedical Engineering·Nianzhen LiAlbert Folch
Jun 26, 2007·International Journal of Biological Sciences·Revathi Ananthakrishnan, Allen Ehrlicher
Dec 25, 2012·Journal of Mathematical Biology·Y SakamotoM H Zaman
Apr 29, 2010·Medical & Biological Engineering & Computing·Jimmy le DigabelBenoit Ladoux
May 21, 2005·Biotechnology Progress·Paul L Gourley
May 26, 2009·Experimental Cell Research·Ying LiuMiriam H Rafailovich
Mar 17, 2010·Biotechnology and Bioengineering·Julianne F AudiffredW Todd Monroe
Oct 19, 2013·Journal of Biomedical Materials Research. Part a·Alicia Calzado-MartínNuria Vilaboa
Dec 30, 2014·Colloids and Surfaces. B, Biointerfaces·Darío Gallach PérezMiguel Manso Silván
May 24, 2005·Biophysical Journal·Muhammad H ZamanDouglas A Lauffenburger
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Darren S GrayChristopher S Chen
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Jan 26, 2007·Journal of Biomedical Materials Research. Part a·J A SzivekS K Williams
Dec 14, 2005·Journal of Biomedical Materials Research. Part a·Herman SahlinJohn A Frangos

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