Micron-sized iron oxide-containing particles for microRNA-targeted manipulation and MRI-based tracking of transplanted cells

Biomaterials
Annekatrin LederIgor M Sauer

Abstract

Particle-based delivery systems for therapeutic manipulation and tracking of transplanted cells by magnetic resonance imaging (MRI) are commonly based on nanometer-sized superparamagnetic iron oxide particles (SPIOs). Here, we present a proof of concept for multifunctional, silica based micron-sized iron oxide-containing particles (sMPIO) that combine fluorescence imaging, MRI tracking, and on-the-spot targeting of specific microRNAs on a particle surface for therapeutic manipulation by RNA interference. Antisense locked nucleic acids (α-LNA) were covalently bound to the surface of silica-based, DAPI-integrated, micron-sized iron oxide particles (sMPIO-α-LNA). In vitro studies using primary human hepatocytes showed rapid particle uptake (4 h) that was accompanied by significant depletion of the targeted microRNA Let7g (80%), up-regulation of the target proteins Cyclin D1 and c-Myc, and specific proteome changes. sMPIO-α-LNA-labeled cells were successfully detected by fluorescence imaging and could be visualized by MRI after intrasplenic transplantation in rats. This new theranostic particle provides a promising tool for cell transplantation where cellular imaging and microRNA-based manipulation is needed. [165].

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Citations

Jun 28, 2015·Advanced Drug Delivery Reviews·Nathanael RaschzokIgor M Sauer
Aug 8, 2015·The British Journal of Radiology·Monique R BernsenGabriel P Krestin
Nov 28, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yongchao MouJin Zhou
Jan 21, 2017·International Journal of Molecular Sciences·Ethel J Ngen, Dmitri Artemov
Oct 21, 2015·Magnetic Resonance Insights·Daniel J KorchinskiJeff F Dunn
Sep 15, 2018·Nanoscale·Melissa Cruz-AcuñaCarlos Rinaldi
Jun 21, 2020·Signal Transduction and Targeted Therapy·Lei LiuShouping Xu
Nov 7, 2019·Annals of Biomedical Engineering·Nasim KiaieHossein Ahmadi Tafti

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