Micro/nano-hierarchical scaffold fabricated using a cell electrospinning/3D printing process for co-culturing myoblasts and HUVECs to induce myoblast alignment and differentiation.

Acta Biomaterialia
Miji Yeo, GeunHyung Kim

Abstract

Human skeletal muscle is composed of intricate anatomical structures, including uniaxially arranged myotubes and widely distributed blood capillaries. In this regard, vascularization is an essential part of the successful development of an engineered skeletal muscle tissue to restore its function and physiological activities. In this paper, we propose a method to obtain a platform for co-culturing human umbilical vein endothelial cells (HUVECs) and C2C12 cells using cell electrospinning and 3D bioprinting. To elaborate, on the surface of mechanical supporters (polycaprolactone and collagen struts) with a topographical cue, HUVECs-laden alginate bioink was uniaxially electrospun. The electrospun HUVECs showed high cell viability (90%), homogeneous cell distribution, and efficient HUVEC growth. Furthermore, the myoblasts (C2C12 cells), which were seeded on the vascularized structure (HUVECs-laden fibers), were co-cultured to facilitate myoblast regeneration. As a result, the scaffold that included myoblasts and HUVECs represented a high degree of the myosin heavy chain (MHC) with striated patterns and enhanced myogenic-specific gene expressions (MyoD, troponin T, MHC and myogenin) as compared to the scaffold that included only my...Continue Reading

Citations

Oct 6, 2020·Tissue Engineering. Part B, Reviews·Jacqueline Clark Ludwig, Barry Andrew Trimmer
Jan 12, 2021·Bio-design and Manufacturing·Pengju WangHaitao Liu
Feb 8, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Noah Z LairdAliasger K Salem
Feb 26, 2021·Journal of Tissue Engineering·Juan M Fernández-CostaJavier Ramón-Azcón
Apr 10, 2021·APL Bioengineering·Muhammad Anwaar NazeerSeda Kizilel
May 1, 2021·Polymers·María Puertas-BartoloméLuis García-Fernández
Jul 15, 2021·APL Bioengineering·Cooper BlakeRichard J Williams
Aug 4, 2021·Essays in Biochemistry·Anita QuigleyRobert M I Kapsa

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