Microneedle-based skin patch for blood-free rapid diagnostic testing

Microsystems & Nanoengineering
Xue Jiang, Peter B Lillehoj

Abstract

Rapid diagnostic tests are one of the most commonly used tests to detect and screen for infectious diseases in the developing world. While these tests are simple, inexpensive, and readily available, they rely on finger-prick blood sampling, which requires trained medical personnel, poses risks of infection, and can complicate cooperation in young children, asymptomatic individuals, and communities with blood taboos. Here, we report a novel microneedle-based skin patch for the rapid detection of protein biomarkers in dermal interstitial fluid. Sample collection is facilitated by a hydrophilic hollow microneedle array that autonomously extracts and transports interstitial fluid to an antibody-based lateral flow test strip via surface tension for colorimetric antigen detection. We employ a simple gold enhancement treatment to enhance the detection sensitivity of this colloidal gold-based lateral flow assay and elucidate the underlying mechanism of this enhancement mechanism through experimental investigation. For proof-of-concept, this device was used to detect Plasmodium falciparum histidine-rich protein 2, a biomarker for malaria infection, which could be detected at concentrations as low as 8 ng/mL. Each test can be completed i...Continue Reading

References

Feb 14, 2004·The Journal of Investigative Dermatology·Nina OtbergJürgen Lademann
Apr 15, 2004·Nature Reviews. Microbiology·David MabeyMark D Perkins
Mar 2, 2005·Diabetes Technology & Therapeutics·Ping M WangMark R Prausnitz
Jan 26, 2007·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Ute JacobiJuergen Lademann
Sep 25, 2007·Proteomics·Jeroen KoolAlbert J R Heck
Aug 22, 2008·The Clinical Journal of Pain·Harvinder S GillMark R Prausnitz
Mar 18, 2011·The Analyst·Joshua Ray WindmillerJoseph Wang
May 12, 2012·Advanced Drug Delivery Reviews·Yeu-Chun KimMark R Prausnitz
May 29, 2012·Malaria Journal·Andrew O FungTheodore B Moore
Aug 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Krishnamurthy V NemaniBarjor Gimi
Sep 17, 2013·Advanced Healthcare Materials·Michael A InvernaleDaniel G Anderson
Oct 7, 2015·Advanced Healthcare Materials·Letizia VentrelliGiuseppe Barillaro
Nov 17, 2016·Journal of Clinical Microbiology·Wellington A OyiboWilliam Brieger
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Pradnya P Samant, Mark R Prausnitz
Jan 21, 2020·Advanced Healthcare Materials·Rongyan HeFei Li

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Citations

Mar 2, 2021·Advanced Healthcare Materials·Hazhir TeymourianJoseph Wang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biosensing

Software Mentioned

MATLAB
AutoCAD
Windows Live Movie Maker

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