Micronized acellular dermal matrix as an efficient expansion substrate and delivery vehicle of adipose-derived stem cells for vocal fold regeneration

The Laryngoscope
Qiang LiangYan Jin

Abstract

Cell therapy has been shown to prevent vocal fold scarring and atrophy. However, problems that include the expansion of large numbers of cells in vitro and the poor survival of transplanted cells in vivo must be solved. The aim of this study was to use micronized acellular dermal matrix (MADM) as an expansion substrate of rabbit allogeneic adipose-derived stem cells (ADSCs) and to apply the combination of the matrix and cells, ADSC-MADM, to vocal fold regeneration. Animal experiment. The proliferation of ADSCs that were cultured on the MADM was evaluated using 3-(4,5-dimethylthizazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium, and a rabbit acute vocal fold injury model was established by laser injury. Eighteen New Zealand white rabbits were randomly divided into three groups, which were injected with ADSC-MADM, ADSCs, and MADM, respectively. Morphological analysis was performed by laryngoscope, and histological analyses were indicated by hematoxylin and eosin staining, van Gieson staining, and immunofluorescence. Additionally, the in vivo survival of the ADSCs was determined by CM-Dil cell labeling. When compared with a two-dimensional culture, the MADM significantly promoted proliferation of ADSCs. Morphol...Continue Reading

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Citations

Apr 15, 2016·The Laryngoscope·Guillaume de BonnecazeValerie Planat-Benard
Mar 10, 2016·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Travis L ShibaJennifer L Long
Mar 6, 2019·The Laryngoscope·Srinivasa Rao NagubothuKatarina Le Blanc
Oct 10, 2018·The Annals of Otology, Rhinology, and Laryngology·Alexander N GoelJennifer L Long
Sep 25, 2017·The Laryngoscope·Tsuyoshi MorisakiShigeru Hirano
Jul 3, 2021·Journal of Clinical Medicine·Reza KaboodkhaniFeridoun Karimi-Busheri
Oct 29, 2021·Current Opinion in Otolaryngology & Head and Neck Surgery·Jacqui Allen

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