PMID: 7542178Jul 1, 1995Paper

Micronuclei induced by modulators of methylation: analogs of 5-azacytidine

Carcinogenesis
H StopperW J Caspary

Abstract

Jones and coworkers demonstrated a qualitative correlation between 5-azacytidine and some of its analogs in inducing changes in cell morphology and their ability in preventing DNA methylation. Previously, we evaluated the same compounds to determine their ability to induce trifluorothymidine (TFT) resistance in L5178Y mouse cells and found that their mutagenic potency also correlated with their reported ability to induce morphological changes in C3H10T1/2 cells. Here, we examine four of the same analogs, 5-fluoro-2'-deoxycytidine, 5-azacytidine, 5,6-dihydro-5-azacytidine and 6-azacytidine, to find out if micronuclei induced by these compounds correlated with these effects. The most cytotoxic analog was 5-fluoro-2'-deoxycytidine, followed by 5-azacytidine. 5,6-Dihydro-5-azacytidine and 6-azacytidine were substantially less cytotoxic. All four compounds induced micronuclei. The lowest dose ranges at which responses were observed for micronucleus induction were -0.04 microM for 5-fluoro-2'-deoxycytidine, 0.2 microM for 5-azacytidine and 10-20 microM for 5,6-dihydro-5-azacytidine and 6-azacytidine. Lack of kinetochore staining in most of the micronuclei indicated that all four compounds were clastogenic. We note a general trend in ...Continue Reading

Citations

Mar 16, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T S DaviesE R Nestmann
Mar 1, 2002·Toxicology Letters·R A Ettlin, D E Prentice
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H Stopper, S O Müller
Feb 18, 2006·Nature Reviews. Drug Discovery·Christine B Yoo, Peter A Jones
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tara S BartonBarbara F Hales
Feb 5, 2008·Journal of Toxicology and Environmental Health. Part a·Cetin SaatciMunis Dundar
Jan 13, 1999·The Journal of Cell Biology·T HaafE I Golub
Nov 22, 2008·Mutation Research·V Zareena Hamza, Mary N Mohankumar
Nov 21, 2009·Differentiation; Research in Biological Diversity·V LabovskyN A Chasseing
Jan 1, 1996·Environmental and Molecular Mutagenesis·D L SpencerK R Tindall
Nov 7, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Steven M BryceStephen D Dertinger
Oct 29, 2003·Journal of Andrology·Tamara L J KellyJacquetta M Trasler
Mar 3, 2006·Cancer Research·Carlo StresemannFrank Lyko
Aug 30, 2021·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Caroline BullMichael Fenech

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.