Micronucleus induction by metronidazole in rat vaginal mucosa

Environmental and Molecular Mutagenesis
José Manuel Ornelas-AguirreG Zúñiga-González

Abstract

Metronidazole (MTZ) is used for the treatment of many infectious diseases, including vaginal infections. While data indicate that MTZ is mutagenic and induces micronuclei in rodents, there is no information on the genotoxicity of MTZ in epithelial vaginal cells or cervical cells. In the present study, we have instilled MTZ into the vagina of rats and evaluated the micronucleus (MN) frequency in proestrus rat vaginal mucosal cells. The first identified proestrus before treatment was used to establish basal proestrus micronucleated cell (PMNC) frequencies. Rats then were assigned to one of five groups: a negative control, three MTZ treatment groups (30, 50, or 100 mg/kg MTZ), and a positive control treated with 2.5 mg of 5-fluorouracil (5-Fu) per rat. Following treatment for five consecutive days, vaginal cell samples were taken daily until three cycles of estrus were completed. Smears prepared from the samples were evaluated for micronuclei in proestrus cells. No differences were found between the PMNC frequencies of the negative control and the 30 and 50 mg/kg MTZ groups. The group treated with 100 mg/kg MTZ, however, had significantly elevated PMNC frequencies in the first and second proestrus samples, while 5-Fu treatment pro...Continue Reading

References

Jan 1, 1978·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·I Morgan
Feb 1, 1976·Journal of the National Cancer Institute·W T SpeckH S Rosenkranz
Dec 1, 1992·Mutation Research·R ForsterJ Westendorf
Jan 1, 1992·Environmental and Molecular Mutagenesis·A Rodríguez-ArizaC Pueyo
May 1, 1991·Mutation Research·Y NagaeH Shimizu
Mar 1, 1984·The Journal of Antimicrobial Chemotherapy·A A Visser, H K Hundt
Jun 1, 1984·The Journal of Surgical Research·A M A-KareemR A Brown
Jan 23, 1981·Science·R L KockP Goldman
Sep 13, 1996·Mutation Research·G ElizondoP Ostrosky-Wegman
Sep 2, 1999·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A ManeoC Mangioni
Aug 24, 2001·American Journal of Obstetrics and Gynecology·R L GoldenbergC Macpherson
Aug 25, 2001·The New England Journal of Medicine·M A KlebanoffUNKNOWN National Institute of Child Health and Human Development Network of Maternal-Fetal Medicine Units
Mar 23, 2002·Mutation Research·Carlos H Leal-GarzaElva I Cortés-Gutiérrez
Jun 8, 2002·Mutation Research·Andrés BendeskyPatricia Ostrosky-Wegman
Jan 23, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M M López NigroM A Carballo
Apr 18, 2003·Archives of Medical Research·Guillermo M Zúñiga-GonzálezApolinar López-Uribe
Jun 13, 2003·Environmental and Molecular Mutagenesis·Patricia GuzmánMaría E Gonsebatt
Dec 16, 2003·Environmental and Molecular Mutagenesis·G Zúñiga-GonzálezO Torres-Bugarín
Feb 7, 2004·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·J L DalrympleL H Smith
May 6, 2004·Basic & Clinical Pharmacology & Toxicology·Abeer F el-Nahas, Ibrahim M el-Ashmawy
Jul 8, 2005·Archives of Gynecology and Obstetrics·Eddie F C MurtaPaulo J Maluf

❮ Previous
Next ❯

Citations

Dec 18, 2010·Mutagenesis·Takeshi MoritaMakoto Hayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.