Microorganism gram-type differentiation based on pyrolysis-mass spectrometry of bacterial Fatty Acid methyl ester extracts.

Applied and Environmental Microbiology
F BasileT L Hadfield

Abstract

Curie-point pyrolysis (Py)-mass spectrometry has been used to differentiate 19 microorganisms by Gram type on the basis of the methyl esters of their fatty acid distribution. The mass spectra of gram-negative microorganisms were characterized by the presence of palmitoleic acid (C(inf16:1)) and oleic acid (C(inf18:1)), as well as a higher abundance of palmitic acid (C(inf16:0)) than pentadecanoic acid (C(inf15:0)). For gram-positive microorganisms, a signal of branched C(inf15:0) (isoC(inf15:0) and/or anteisoC(inf15:0)) more intense than that of palmitic acid was observed in the mass spectra. Principal components analysis of these mass spectral data segregated the microorganisms investigated in this study into three discrete clusters that correlated to their gram reactions and pathogenicities. Further tandem mass spectrometric analysis demonstrated that the nature of the C(inf15:0) fatty acid isomer (branched or normal) present in the mass spectrum of each microorganism was important for achieving the classification into three clusters.

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Citations

Jul 14, 2007·Journal of Mass Spectrometry : JMS·Mohammed A MeetaniFranco Basile
Dec 19, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Sophie E Lellman, Rainer Cramer
May 16, 2019·Microorganisms·Ricardo Franco-DuarteCélia F Rodrigues
Jun 11, 1999·Analytical Chemistry·R J ArnoldJ P Reilly
Apr 29, 2006·Analytical Chemistry·Jeffrey J JonesRichard M O'Brien
Feb 1, 1996·Current Opinion in Biotechnology·R Goodacre, D B Kell
Aug 10, 1999·Journal of the American Society for Mass Spectrometry·M B BeverlyT L Hadfield
Nov 3, 2004·Journal of the American Society for Mass Spectrometry·Jeffrey J JonesCharles L Wilkins
Mar 29, 2007·Journal of Proteome Research·Shaofeng Zhang, Franco Basile

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