Microorganisms degrading chlorobenzene via a meta-cleavage pathway harbor highly similar chlorocatechol 2,3-dioxygenase-encoding gene clusters

Archives of Microbiology
Markus GöbelW Reineke

Abstract

Pseudomonas putida GJ31 harbors a degradative pathway for chlorobenzene via meta-cleavage of 3-chlorocatechol. Pseudomonads using this route for chlorobenzene degradation, which was previously thought to be generally unproductive, were isolated from various contaminated environments of distant locations. The new isolates, Pseudomonas fluorescens SK1 (DSM16274), Pseudomonas veronii 16-6A (DSM16273), Pseudomonas sp. strain MG61 (DSM16272), harbor a chlorocatechol 2,3-dioxygenase (CbzE). The cbzE-like genes were cloned, sequenced, and expressed from the isolates and a mixed culture. The chlorocatechol 2,3-dioxygenases shared 97% identical amino acids with CbzE from strain GJ31, forming a distinct family of catechol 2,3-dioxygenases. The chlorocatechol 2,3-dioxygenase, purified from chlorobenzene-grown cells of strain SK1, showed an identical N-terminal sequence with the amino acid sequence deduced from cloned cbzE. In all investigated chlorobenzene-degrading strains, cbzT-like genes encoding ferredoxins are located upstream of cbzE. The sequence data indicate that the ferredoxins are identical (one amino acid difference in CbzT of strain 16-6A compared to the others). In addition, the structure of the operon downstream of cbzE is ...Continue Reading

Citations

Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Oct 26, 2005·Applied Microbiology and Biotechnology·Michael BramucciVasantha Nagarajan
Mar 27, 2007·Applied Microbiology and Biotechnology·Francesca RadicePaola Barbieri
May 27, 2008·Journal of Bacteriology·Mariëlle J H MoonenWillem J H van Berkel
Jul 29, 2015·Applied Microbiology and Biotechnology·Martina Kiel, Karl-Heinrich Engesser
Mar 5, 2014·Microbial Cell Factories·Pankaj Kumar Arora, Hanhong Bae
Aug 19, 2014·Microbial Biotechnology·Daniel Dobslaw, Karl-Heinrich Engesser
Apr 2, 2021·Environmental Science and Pollution Research International·Roger Jay De VelaPeter Alan Gostomski

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