Microparticle formation after exposure of blood to activated endothelium under flow

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Marion G MaceyCharlotte Lawson

Abstract

Increased numbers of circulating microparticles (MPs) are indicative of poor clinical outcome in a number of inflammatory disorders, including atherosclerosis. Platelets and megakaryocytes are a major source of MP and are identified by presence of CD42b on the MP surface. MP shed from activated platelets can be identified by presence of P-selectin (CD62P). Tissue factor (TF) is the principal initiator of blood coagulation and its activity has been identified in MPs derived from patient plasma, which may contribute to thrombosis. Here, we have investigated by flow cytometry the expression of TF and CD62P on MP after exposure of diluted whole blood to TNF-activated endothelial cells (EC) both under static conditions and in our newly established model of flow. MPs were significantly increased in blood subjected to flow and this was further enhanced after exposure of blood to TNF-activated EC. MP surface expression of CD62P or TF was upregulated following exposure to TNF-activated EC under flow compared with flow with nonactivated EC or after static coculture with and without prior EC activation. These data strongly suggest that interactions of blood with inflamed EC can modulate production of CD62P and TF bearing MP under flow con...Continue Reading

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Citations

Jun 22, 2011·Basic Research in Cardiology·Silvia Montoro-GarcíaGregory Y H Lip
Apr 19, 2013·European Journal of Clinical Investigation·Stefan WeisshaarMichael Wolzt
Apr 21, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Attila Tárnok
Jul 3, 2013·Journal of Veterinary Internal Medicine·J M HerringS A Smith
Jan 4, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Emma HoltomCharlotte Lawson
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·D Varon, E Shai
Sep 8, 2016·Biochimica Et Biophysica Acta·Camille EttelaieAnthony Maraveyas
Dec 17, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Ela Shai, David Varon
Apr 22, 2015·Cellular and Molecular Life Sciences : CMLS·Dimitry A ChistiakovYuri V Bobryshev

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