Microparticle Release from Cell Lines and Its Anti-Influenza Activity

Viral Immunology
Saharat JantaratriratPrasert Auewarakul

Abstract

Microparticles (MPs) are vesicles that are released by budding from plasma membrane of living cells. Recently, the role of MPs in antiviral activity has been proposed. We investigated quantity and anti-influenza activity of MPs from human alveolar epithelial cells A549, human bronchial epithelial cells BEAS-2B, human colon adenocarcinoma cells HT-29, and the human lung fibroblast cells MRC-5. MPs were found from all four cell lines. However, anti-influenza activity against an H1N1 influenza virus was found only from MPs of A549 and BEAS-2B. BEAS-2B cell differentiation did not increase MP release. Methyl-β-cyclodextrin (MβCD) increased MP release and anti-influenza activity in HT-29 and A549. MP release increased after calcium ionophore A23187 treatment in three cell lines but only in HT-29 after forskolin treatment. These findings provide in vitro data supporting the role of MPs as an innate defense against influenza virus and as an approach to enhance the defense.

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Citations

Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Yuan JiangChuanshan Xu
May 23, 2021·Advanced Drug Delivery Reviews·Anna Lena JungBirke J Benedikter

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Methods Mentioned

BETA
bronchoalveolar lavage
ELISA
flow cytometry
electron microscopy
FACS

Software Mentioned

CellQuest

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