Microparticles from β-thalassaemia/HbE patients induce endothelial cell dysfunction

Scientific Reports
Wasinee KheansaardSaovaros Svasti

Abstract

Thromboembolic complication occurs frequently in β-thalassaemia/HbE patients, particularly in splenectomised patients. Endothelial cells play an important role in thrombosis. There is strong evidence of endothelial cell activation and dysfunction in β-thalassaemia. Microparticles (MPs) are associated with thrombosis and endothelial cell dysfunction in many diseases including β-thalassaemia. However, the effect of thalassaemic-MPs on endothelial cells mediating thrombus formation has not been elucidated. In this study, the effects of circulating MPs from β-thalassaemia/HbE patients on endothelial cell functions were investigated. The results showed that MPs directly induce tissue factor, interleukin (IL)-6, IL-8, intracellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin expression in human umbilical vein endothelial cells (HUVECs). Notably, the levels of these endothelial cell activation markers were significantly increased in HUVECs treated with MPs obtained from splenectomised β-thalassaemia/HbE patients when compared to MPs from non-splenectomised patients or normal subjects. The increased endothelial cell activation ultimately lead to increased monocyte-endothelial cell adhesion. THP-1 and HUVECs ad...Continue Reading

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Citations

Dec 31, 2020·International Journal of Molecular Sciences·Kiruphagaran ThangarajuPaul W Buehler

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Methods Mentioned

BETA
confocal microscopy
ELISA
flow cytometry

Software Mentioned

SPSS
CellQuestPro TM
NIS Element Viewer
GraphPad PRISM

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