Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation.

Journal of the American College of Surgeons
Ritha M BelizaireTimothy A Pritts

Abstract

Transfusion of stored blood is associated with increased complications. Microparticles (MPs) are small vesicles released from RBCs that can induce cellular dysfunction, but the role of RBC-derived MPs in resuscitation from hemorrhagic shock is unknown. In the current study, we examined the effects of RBC-derived MPs on the host response to hemorrhage and resuscitation. MPs were isolated from murine packed RBC units, quantified using flow cytometry, and injected into healthy mice. Separate groups of mice underwent hemorrhage and resuscitation with and without packed RBC-derived MPs. Lungs were harvested for histology and neutrophil accumulation and assessed by myeloperoxidase content. Human neutrophils were treated with human RBC-derived MPs and CD11b expression, superoxide production, and phagocytic activity were determined. Stored murine packed RBC units contained increased numbers of RBC-derived MPs compared with fresh units. Hemorrhaged mice resuscitated with MPs demonstrated substantially increased pulmonary neutrophil accumulation and altered lung histology compared with mice resuscitated without MPs. Intravenous injection of MPs into normal mice resulted in neutrophil priming, evidenced by increased neutrophil CD11b expre...Continue Reading

References

Jul 31, 1998·Clinical Science·A M CondliffeE R Chilvers
Dec 20, 1999·Transfusion Medicine Reviews·C F Högman, H T Meryman
Feb 12, 2000·American Journal of Surgery·G ZallenC C Silliman
Jun 7, 2002·Archives of Surgery·Patrick J OffnerChristopher C Silliman
Nov 24, 2005·Antimicrobial Agents and Chemotherapy·M T LabroC Babin-Chevaye
Apr 16, 2008·Journal of Internal Medicine·A S LeroyerC M Boulanger
Jul 9, 2010·Shock·Kevin R KastenCharles C Caldwell
Jul 14, 2010·The Journal of Thoracic and Cardiovascular Surgery·Albert H M van StratenVolkher Scharnhorst
Oct 30, 2010·Circulation Research·Sebastian F Mause, Christian Weber
Dec 15, 2010·The Journal of Trauma·Jordan A WeinbergLoring W Rue
May 12, 2011·Cellular and Molecular Life Sciences : CMLS·Bence GyörgyEdit I Buzás
Aug 20, 2011·Circulation Research·Pierre-Emmanuel RautouChantal M Boulanger
Oct 25, 2011·Critical Care : the Official Journal of the Critical Care Forum·Ramaroson Andriantsitohaina
Feb 10, 2012·The Journal of Surgical Research·Amy T MakleyTimothy A Pritts

❮ Previous
Next ❯

Citations

May 3, 2013·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Walter G Land
Jul 3, 2013·American Journal of Respiratory Cell and Molecular Biology·Viola DenglerEric P Schmidt
Dec 11, 2014·The Journal of Trauma and Acute Care Surgery·Satbir K DhillonMyung S Park
Jan 11, 2013·Current Opinion in Anaesthesiology·Rachael P Jackman
Nov 26, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Thierry BurnoufJerard Seghatchian
May 3, 2014·Transfusion Medicine·M W KentC C Silliman
Jun 27, 2014·Journal of Cardiac Surgery·Abdallah K AlameddineJohn A Rousou
Apr 17, 2014·Transfusion·Elliott Bennett-GuerreroTimothy J McMahon
Nov 23, 2015·Journal of Thrombosis and Thrombolysis·Grigory LevinAthina Lavrentieva
Jul 17, 2014·American Journal of Respiratory Cell and Molecular Biology·Eleftheria LetsiouJoe G N Garcia
Dec 24, 2014·American Journal of Veterinary Research·Stephanie A SmithMaureen McMichael
Oct 4, 2016·Transfusion·Jennifer A MuszynskiUNKNOWN Pediatric Critical Care Blood Research Network (Blood Net)
Jun 12, 2016·Thorax·Sanooj SoniMasao Takata
Mar 24, 2017·The Journal of Trauma and Acute Care Surgery·Sanjna ShahJennifer A Muszynski
Jun 18, 2017·International Journal of Molecular Sciences·Maike E van HezelNicole P Juffermans
Sep 1, 2018·Transfusion·Jason P AckerElisabeth Maurer-Spurej
Jan 8, 2019·The Journal of Trauma and Acute Care Surgery·Grace E MartinMichael D Goodman
Mar 21, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mark J McVeyWolfgang M Kuebler
Jul 20, 2019·Transfusion·Somaang Menocha, Jennifer A Muszynski
May 1, 2019·Journal of Cardiovascular Pharmacology and Therapeutics·Ashish K RehniKunjan R Dave
May 16, 2019·The Journal of Cardiovascular Surgery·Hisato TakagiUNKNOWN ALICE (All-Literature Investigation of Cardiovascular Evidence) Group
Sep 16, 2018·Blood Advances·Ruqayyah J AlmizraqJennifer A Muszynski
Mar 10, 2020·Bioscience Reports·Li YangYuanshuai Huang
Mar 25, 2015·Biological Chemistry·Richard S HoehnTimothy A Pritts
Jun 27, 2020·The Journal of Trauma and Acute Care Surgery·Kasiemobi E PulliamTimothy A Pritts
Feb 27, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nadine BeckmannTimothy A Pritts
May 24, 2015·The Journal of Trauma and Acute Care Surgery·Philip C SpinellaAndrew P Cap
Nov 30, 2016·Frontiers in Immunology·Nayef M KazzazJason S Knight

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.