Microparticles of healthy origins improve endothelial progenitor cell dysfunction via microRNA transfer in an atherosclerotic hamster model

Acta Physiologica
Nicoleta AlexandruAdriana Georgescu

Abstract

In this study, we aimed: (i) to obtain and functionally characterize the cultures of late endothelial progenitor cells (EPCs) from the animal blood; (ii) to investigate the potential beneficial effects of circulating microparticles (MPs) of healthy origins on EPC dysfunctionality in atherosclerosis as well as involved mechanisms. Late EPCs were obtained and expanded in culture from peripheral blood isolated from two animal groups: hypertensive-hyperlipidaemic (HH) and control (C) hamsters. In parallel experiments, late EPC cultures from HH were incubated with MPs from C group. The results showed that late EPCs display endothelial cell phenotype: (i) have ability to uptake 1,1-dioctadecyl-3,3,3,3 tetramethylindocarbocyanine-labelled acetylated low-density lipoprotein and Ulex europaeus agglutinin lectin-1; (ii) express CD34, CD133, KDR, CD144, vWF, Tie-2. Late EPCs from HH exhibited different morphological and functional characteristics compared to control: (i) are smaller and irregular in shape; (ii) present decreased endothelial surface marker expression; (iii) display reduced proliferation, migration and adhesion; (iv) lose ability to organize themselves into tubular structures and integrate into vascular network; (v) have di...Continue Reading

References

Jan 5, 2000·The Journal of Clinical Investigation·Y LinR P Hebbel
Feb 10, 2000·Circulation Research·A Bayes-GenisR S Schwartz
May 29, 2002·Experimental Hematology·Monika Baj-KrzyworzekaMariusz Z Ratajczak
Oct 4, 2003·Blood·Ferdinand H BahlmannDanilo Fliser
Dec 31, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Jin HurYoung-Bae Park
May 8, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Wolfram ScharbrodtJohannes Wiecha
Jul 17, 2004·Current Opinion in Hematology·Olivier MorelJean-Marie Freyssinet
Jan 18, 2005·Physiology·Bénédicte HugelJean-Marie Freyssinet
Jan 22, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Nicolle KränkelRainer Hambrecht
Sep 9, 2005·The New England Journal of Medicine·Nikos WernerGeorg Nickenig
Mar 30, 2007·Leukemia·D N PraterM C Yoder
Sep 14, 2007·Cardiovascular Research·Marta DíezVictor I Peinado
Nov 10, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Chao-Hung WangWilliam L Stanford
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tamia A HarrisCharles J Lowenstein
Feb 12, 2008·Journal of the American College of Cardiology·Daniel P SievekingMartin K C Ng
May 31, 2008·Expert Review of Cardiovascular Therapy·Glenn Marsboom, Stefan Janssens
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 14, 2008·Cardiovascular Research·Carmen UrbichStefanie Dimmeler
Oct 24, 2008·Journal of Vascular Surgery·Shirling TsaiK Craig Kent
Jul 17, 2009·Critical Care : the Official Journal of the Critical Care Forum·Tian Hang LuoGuo En Fang
Aug 27, 2009·The Journal of Biological Chemistry·Shimin DongChunxiang Zhang
Jan 21, 2010·Microvascular Research·Toyoaki Murohara
Jun 18, 2010·Investigative Ophthalmology & Visual Science·Reinhold J MedinaAlan W Stitt
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yun FangPeter F Davies
Aug 17, 2010·Ageing Research Reviews·Li-Hsin ChenShih-Hwa Chiou
Oct 22, 2010·Journal of Thrombosis and Haemostasis : JTH·N AlexandruA Georgescu
Jan 21, 2011·Molecular and Cellular Biochemistry·M BalasubramanyamV Mohan
Feb 18, 2011·Journal of Cellular Biochemistry·Min YuXuerui Tan
Jul 22, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Min YuXuerui Tan
Dec 3, 2011·Cardiovascular Research·Philipp Jakob, Ulf Landmesser
Jan 12, 2012·Journal of Endocrinological Investigation·S La VigneraA E Calogero
Feb 2, 2012·Journal of Cell Science·Thalyana Smith-Vikos, Frank J Slack

❮ Previous
Next ❯

Citations

Feb 15, 2019·Acta Physiologica·Philipp HillmeisterAnja Bondke Persson
Oct 3, 2019·Journal of Cellular and Molecular Medicine·Zeyu ShuQiang Zhang
Oct 31, 2019·Journal of Cardiovascular Translational Research·Mengna PengGelin Xu
May 10, 2019·Frontiers in Pharmacology·Nicoleta AlexandruAdriana Georgescu
Dec 19, 2017·Acta Physiologica·A ZietzerF Jansen
Jun 19, 2021·Reviews in Endocrine & Metabolic Disorders·Nicoleta AlexandruAdriana Georgescu
Jul 13, 2021·Ophthalmic Genetics·Huijuan XuZhenglin Yang
Sep 14, 2021·Frontiers in Cardiovascular Medicine·Cai-Yu ZengYuan-Qiang Lu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.