Micropatterned biodegradable polyesters clicked with CQAASIKVAV promote cell alignment, directional migration, and neurite outgrowth

Acta Biomaterialia
Deteng ZhangChangyou Gao

Abstract

The interplay of microstructures and biological cues is critical to regulate the behaviors of Schwann cells (SCs) in terms of cellular spatial arrangement and directional migration as well as neurite orientation for bridging the proximal and distal stumps of the injured peripheral nervous system. In this study, stripe micropatterns having ridges/grooves of width 20/20 and 20/40 μm were fabricated on the surface of maleimide-functionalized biodegradable poly(ester carbonate) (P(LLA-MTMC)) films by the polydimethylsiloxane mold-pressing method, respectively. The laminin-derived CQAASIKVAV peptides end-capped with an SH group were then grafted by the thiol-ene click reaction under mild conditions to obtain micropatterned and peptide-grafted films. SCs cultured on these films, especially on the 20/40-μm film, displayed faster and aligned adhesion as well as a larger number of elongated cells with a higher length-to-width (L/W) ratio along the stripe direction than those on the flat-pep film. The migration rate of SCs was significantly enhanced in parallel to the stripe direction with a large net displacement. The micropatterned and peptide-grafted films, especially the 20/40-μm film, could promote SC proliferation and nerve growth ...Continue Reading

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Citations

Feb 15, 2020·Macromolecular Bioscience·Deteng ZhangChangyou Gao
Aug 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiayu LiuXiao-Bing Zhang
Apr 6, 2020·Biomaterials Science·Liangliang YangPatrick van Rijn
Mar 21, 2020·Advanced Science·Shida MiaoLijie Grace Zhang
May 18, 2021·Bioactive Materials·Wen XueBin Duan
Jul 3, 2021·Burns and Trauma·Yulan HuangMaling Gou
Jul 25, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ying MaXiaodong Cao

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