Micropatterned immobilization of epidermal growth factor to regulate cell function

Bioconjugate Chemistry
Y ItoY Imanishi

Abstract

Photoreactive epidermal growth factor (EGF) was synthesized by conjugating mouse EGF with photoreactive polyallylamine, which was synthesized by the coupling reaction of polyallylamine with N-[4-(azidobenzoyl)oxy]succinimide. The EGF derivative was pattern-immobilized onto a polystyrene plate by UV irradiation in the presence of a photomask in a prescribed micropattern. The patterned immobilization of EGF on the polystyrene plate was confirmed by immunostaining with anti-EGF antibody. Chinese hamster ovary (CHO) cells overexpressing EGF receptors were cultured on the micropatterned plate. The phosphorylated tyrosine residues of signal proteins, including EGF receptors, were detected only in the cells adhered in the EGF-immobilized area, and cell growth was observed only in the EGF-immobilized area. The cells growing in the EGF-immobilized area were partially stained by anti-phosphotyrosine antibody, when the area of EGF immobilization was smaller than the cell. The partial staining of activated proteins indicates that immobilization of EGF inhibited the free lateral diffusion and internalization of the activated EGF-EGF receptor complex. The enhanced cell growth is due to juxtacrine stimulation realized by immobilized EGF.

References

Jan 1, 1993·Annual Review of Biochemistry·J Massagué, A Pandiella

❮ Previous
Next ❯

Citations

Oct 14, 2008·Annals of Biomedical Engineering·Tracy Jane Stefonek-Puccinelli, Kristyn S Masters
Dec 11, 2008·Annals of Biomedical Engineering·Leandro ForcinitiMuhammad H Zaman
Oct 20, 2005·Journal of Bioscience and Bioengineering·Hiroshi MakinoYoshihiro Ito
Nov 16, 2012·Tissue Engineering. Part B, Reviews·Silviya P ZustiakJennie B Leach
Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
Jul 5, 2001·Journal of Biomaterials Science. Polymer Edition·Y ItoY Imanishi
Apr 30, 2003·Zoological Science·Kohei WatanabeRyoichi Matsuda
Apr 20, 2010·Acta Biomaterialia·Tracy J PuccinelliKristyn S Masters
Dec 17, 2008·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Claudia BroseMeike Reimann
May 20, 2008·Journal of Neuroscience Methods·Laura M Y YuMolly S Shoichet
Aug 9, 2007·Colloids and Surfaces. B, Biointerfaces·Zuwei MaChangyou Gao
Nov 23, 2006·Journal of Bioscience and Bioengineering·Tomohiro KonnoYoshihiro Ito
Dec 11, 2007·Journal of Biomedical Materials Research. Part a·Claude LeclercRichard Lo-Man
Nov 22, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Tracy Jane Stefonek, Kristyn S Masters
Dec 16, 2008·IEEE Reviews in Biomedical Engineering·Jay W WarrickDavid J Beebe
Apr 8, 2006·Biomaterials·Constant VodouhêPhilippe Lavalle
Jul 19, 2016·International Journal of Biological Macromolecules·Haram LeeHansoo Park
Sep 2, 1998·Journal of Biomaterials Science. Polymer Edition·Y ItoY Imanishi
Nov 5, 2005·Journal of Biomaterials Science. Polymer Edition·Evelyn K F Yim, Kam W Leong
Aug 17, 2002·Journal of Biomaterials Science. Polymer Edition·X Cao, M S Shoichet
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Junichiro SarukawaHiroshi Tamura
Oct 3, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Young Kyun WooYong Soon Park
Nov 18, 2006·Journal of Biomedical Materials Research. Part a·Natalia Gomez, Christine E Schmidt
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·Mojgan HeydariYoshihiro Ito
Sep 22, 2010·Macromolecular Bioscience·Loraine L Y ChiuGordana Vunjak-Novakovic
Jan 28, 2014·Advanced Healthcare Materials·Catarina A CustódioJoão F Mano
Apr 22, 2011·Macromolecular Bioscience·Kristyn S Masters
May 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jordi Cabanas-DanésPascal Jonkheijm
Jun 23, 2020·Biotechnology and Bioengineering·Ana M Sandoval-CastellanosJohn W Haycock

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.