Feb 5, 2008

Micropatterning of proteins on the surface of three-dimensional poly(ethylene glycol) hydrogel microstructures

Analytica Chimica Acta
Dae Nyun KimWon-Gun Koh

Abstract

This paper describes micropatterning of proteins on the surface of three-dimensional hydrogel microstructures. Poly(ethylene glycol) (PEG)-based hydrogel microstructures were fabricated on a glass substrate using a poly(dimethylsiloxane) (PDMS) replica as a molding insert and photolithography. The lateral dimension and height of the hydrogel microstructures were easily controlled by the feature size of the photomask and depth of the PDMS replica, respectively. Bovine serum albumin (BSA), a model protein, was covalently immobilized to the surface of the hydrogel microstructure via a 5-azidonitrobenzoyloxy N-hydroxysuccinimide bifunctional linker at a surface density of 1.48 mg cm(-2). The immobilization of BSA on the PEG hydrogel surface was demonstrated with XPS by confirming the formation of a new nitrogen peak, and the selective immobilization of fluorescent-labeled BSA on the outer region of the three-dimensional hydrogel micropattern was demonstrated by fluorescence. A hydrogel microstructure could immobilize two different enzymes separately, and sequential bienzymatic reaction was demonstrated by reacting glucose and Amplex Red with a hydrogel microstructure where glucose oxidase was immobilized on the surface and peroxida...Continue Reading

  • References9
  • Citations18

References

Mentioned in this Paper

Covalent Interaction
Amines
Hydrogen Peroxide
Ethylene Glycol Measurement
Phenyl
Enzymes, antithrombotic
Biosensors
Filamentous fungus
Peroxidases
Surface Properties

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