Microphthalmia and lack of vitreous body in transgenic mice expressing the first immunoglobulin-like domain of nectin-1.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Kazuhiko YoshidaEtsuro Ono

Abstract

Nectins are Ca2+-independent immunoglobulin (Ig)-like cell-cell-adhesion molecules. We have generated transgenic mice expressing a series of soluble forms of nectin-1, and investigated special effects of each soluble form of nectin-1 in vivo. In the course of generating transgenic mice expressing a soluble form of nectin-1 consisting of the first Ig-like domain of nectin-1 and the Fc portion of human IgG1 (PHveC-VhIg), we found that all of the transgenic founder mice showed a microphthalmia. The purpose of this study is to examine functions of the extracellular domains of nectin-1 in eye development using transgenic technology. Eyes of four different transgenic mouse lines expressing each soluble form of nectin-1 were analyzed histologically. Tissue sections were processed with hematoxylin-eosin staining and indirect immunoperoxidase technique. All of five transgenic mouse founders expressing PHveC-VhIg, and of three lines expressing PHveC-VpIg made of the first Ig-like domain fused to porcine Fc portions at 5 weeks showed a microphthalmia, but not all of the transgenic mouse lines expressing PHveCIg or PHveCpIg made of the entire ectodomain fused to human or porcine Fc portions. In the abnormal eyes, the vitreous body was almo...Continue Reading

References

Aug 25, 1999·Anatomy and Embryology·M Ito, M Yoshioka
Jul 18, 2002·Current Biology : CB·Kumi Ozaki-KurodaYoshimi Takai
Nov 29, 2002·Journal of Cell Science·Yoshimi Takai, Hiroyuki Nakanishi
Apr 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Noriko OkabeYoshimi Takai
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Etsuro OnoToshimitsu Uede
Dec 14, 2004·Current Eye Research·Tibor Valyi-NagyDeepak Shukla
Apr 27, 2005·Investigative Ophthalmology & Visual Science·Satoshi TaharaguchiEtsuro Ono

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