Micropipet-based pico force transducer: in depth analysis and experimental verification

Biophysical Journal
D A SimsonRudolf Merkel

Abstract

Measurements of forces in the piconewton range are very important for the study of molecular adhesion and mechanics. Recently, a micropipet-based force transducer for this type of experiment was presented (E. Evans, K. Ritchie, and R. Merkel, 1995, Biophys. J., 68:2580-2587). In the present article we give a detailed mechanical analysis of this transducer, including nonlinear effects. An analytical expression for the transducer stiffness at small elongations is given. Using magnetic tweezers (F. Ziemann, J. Rädler, and E. Sackmann, 1994, Biophys. J., 66:2210-2216), we were able to determine the force displacement relation of this transducer experimentally. Forces from approximately 10 pN to 500 pN were applied. Theoretical predictions and experimental results coincide remarkably well.

References

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Citations

Nov 26, 2010·European Biophysics Journal : EBJ·Christopher BramptonPhilip M Williams
Sep 23, 2011·Journal of the Royal Society, Interface·L SunY W Zhang
Sep 25, 2009·The European Physical Journal. E, Soft Matter·M-J ColbertK Dalnoki-Veress
May 17, 2011·PloS One·Julien HussonNelly Henry
Nov 5, 2013·The Review of Scientific Instruments·R V Seshagiri RaoPramod A Pullarkat
May 1, 2007·Biophysical Journal·Volkmar Heinrich, Chawin Ounkomol
Oct 7, 2015·Interface Focus·Qianxi WangKuo-Kang Liu
Nov 30, 2010·Biophysical Journal·Marie-Josée ColbertKari Dalnoki-Veress
Nov 26, 2010·Journal of Biomechanics·Lu SunYong-Wei Zhang
Dec 24, 2013·Biophysical Journal·Kristian BoyeRudolf Merkel
May 23, 2002·Biophysical Journal·Charlie Gosse, Vincent Croquette
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·E Evans
May 17, 2006·Acta Biomaterialia·Guy LadamErich Sackmann
Sep 30, 2004·Biophysical Journal·Sébastien PierratPierre Nassoy
Sep 22, 2017·Molecular Biology of the Cell·Anna SawickaJulien Husson
Jul 5, 2002·Physical Review Letters·K PrechtelR Merkel
Jan 15, 2014·Physical Chemistry Chemical Physics : PCCP·Sabyasachi Rakshit, Sanjeevi Sivasankar
Mar 16, 2017·BMC Biophysics·Daniel ŠmítMartin Zapotocky
Nov 23, 2007·Biochemical Society Transactions·D J Brockwell
Jan 28, 2010·Nano Letters·Waldemar HällströmChristelle N Prinz
Mar 2, 2011·The Journal of Physical Chemistry. B·H-M LoritzR Merkel

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