Microplate assay measurement of cytochrome p450-carbon monoxide complexes

Journal of Biochemistry and Molecular Biology
Suk-Jung ChoiJoong-Kyun Jeon

Abstract

Cytochrome P450 in microsomes can be quantitated using the characteristic 450 nm absorption peak of the CO adduct of reduced cytochrome P450. We developed a simple microplate assay method that is superior to previous methods. Our method is less laborious, suitable for analyzing many samples, and less sensitive to sample aggregation. Microsome samples in microplate wells were incubated in a CO chamber rather than bubbled with CO gas, and then reduced with sodium hydrosulfite solution. This modification allowed a reliable and reproducible assay by effectively eliminating variations between estimations.

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Citations

Dec 17, 2010·Applied Microbiology and Biotechnology·Rama Krishna Gudiminchi, Martha Sophia Smit
Mar 25, 2011·Toxicology International·D Udaya LakshmiV Vykunta Rao
Apr 25, 2012·Indian Journal of Pharmacology·G Dilip ReddyM Vijay Kumar
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Feb 5, 2013·Insect Biochemistry and Molecular Biology·Minmin SongClaus Tittiger
Aug 21, 2014·Insect Biochemistry and Molecular Biology·Minmin SongClaus Tittiger
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Aug 29, 2021·Environmental Science and Pollution Research International·Kritika PanditSatwinderjeet Kaur

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