Micropost arrays for measuring stem cell-derived cardiomyocyte contractility

Methods : a Companion to Methods in Enzymology
Kevin M BeussmanNathan J Sniadecki

Abstract

Stem cell-derived cardiomyocytes have the potential to be used to study heart disease and maturation, screen drug treatments, and restore heart function. Here, we discuss the procedures involved in using micropost arrays to measure the contractile forces generated by stem cell-derived cardiomyocytes. Cardiomyocyte contractility is needed for the heart to pump blood, so measuring the contractile forces of cardiomyocytes is a straightforward way to assess their function. Microfabrication and soft lithography techniques are utilized to create identical arrays of flexible, silicone microposts from a common master. Micropost arrays are functionalized with extracellular matrix protein to allow cardiomyocytes to adhere to the tips of the microposts. Live imaging is used to capture videos of the deflection of microposts caused by the contraction of the cardiomyocytes. Image analysis code provides an accurate means to quantify these deflections. The contractile forces produced by a beating cardiomyocyte are calculated by modeling the microposts as cantilever beams. We have used this assay to assess techniques for improving the maturation and contractile function of stem cell-derived cardiomyocytes.

References

Jul 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T EschenhagenE L Elson
Apr 8, 1999·European Biophysics Journal : EBJ·J DomkeM Radmacher
Feb 9, 2002·Journal of Biomedical Materials Research·Valerie A LiuSangeeta N Bhatia
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John L TanChristopher S Chen
Apr 19, 2003·Journal of Colloid and Interface Science·Kirill EfimenkoJan Genzer
Nov 10, 2004·Biophysical Journal·Shizhuo YinJoseph Cheung
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olivia du RoureBenoit Ladoux
Nov 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·István Ferenc EdesZoltán Papp
May 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alexandre SaezBenoît Ladoux
Jul 7, 2007·Methods in Cell Biology·Nathan J Sniadecki, Christopher S Chen
Jul 1, 2008·Biophysical Journal·Jeffrey G JacotJeffrey H Omens
Jul 23, 2008·Journal of Biomechanics·Jinseok KimSukho Park
May 19, 2009·Journal of Cellular Biochemistry·Christian Freund, Christine L Mummery
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wesley R LegantChristopher S Chen
Oct 13, 2009·Journal of Biomechanics·Jeffrey G JacotDarlene L Hunt
Jun 16, 2010·Cardiovascular & Hematological Agents in Medicinal Chemistry·Federico MosnaMauro Krampera
Jan 29, 2011·The Biochemical Journal·Cheryl DambrotChristine L Mummery
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·A SaezB Ladoux
May 6, 2011·Molecular Pharmaceutics·Dinender K SinglaBinbin Yan
Nov 15, 2011·Nature Reviews. Drug Discovery·Marica GrskovicGeorge Q Daley
Nov 19, 2011·Tissue Engineering. Part a·Thomas BoudouChristopher S Chen
Mar 15, 2012·European Heart Journal·Eugene Braunwald
Jun 1, 2012·International Journal of Cell Biology·Laurie B HazeltineSean P Palecek
Sep 26, 2012·Biomedical Microdevices·Rebecca E TaylorBeth L Pruitt
Oct 5, 2012·Nature Reviews. Molecular Cell Biology·Milena BellinChristine L Mummery
Feb 2, 2013·Circulation Research·Mark MercolaErik Willems
Dec 24, 2013·Journal of Biomechanics·Jin YouJinseok Kim
Feb 1, 2014·Circulation Research·Xiulan YangCharles E Murry
Feb 20, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·A C C van SpreeuwelC V C Bouten
Feb 25, 2014·Methods in Cell Biology·Nathan J SniadeckiShirin Feghhi
Mar 13, 2014·Journal of Biomechanical Engineering·Marita L RodriguezNathan J Sniadecki
Apr 17, 2014·Journal of Molecular and Cellular Cardiology·Xiulan YangCharles E Murry
Aug 19, 2014·ACS Applied Materials & Interfaces·Alexandre J S RibeiroBeth L Pruitt
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kavitha T KuppusamyHannele Ruohola-Baker
Jan 23, 2007·Soft Matter·Sami Alom Ruiz, Christopher S Chen

❮ Previous
Next ❯

Citations

Sep 1, 2015·Methods : a Companion to Methods in Enzymology·Michael J SiedlikCeleste M Nelson
Feb 21, 2016·Methods : a Companion to Methods in Enzymology·Nicolas BorghiChristophe Lavelle
Mar 30, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Allen P LiuSapun H Parekh
Feb 23, 2018·The Journal of Cell Biology·Florence Broders-BondonEmmanuel Farge
May 31, 2018·Lab on a Chip·Henry H ChungThomas R Gaborski
Apr 12, 2019·Circulation Research·Jason W MiklasDeok-Ho Kim
Jan 18, 2020·Advanced Healthcare Materials·Ava M ObenausNathan J Sniadecki
Apr 10, 2020·Advanced Healthcare Materials·Cheavar A Blair, Beth L Pruitt
May 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Paola ManiniMarco d'Ischia
Oct 13, 2019·Nature Communications·Jason W MiklasHannele Ruohola-Baker
Sep 3, 2020·International Journal of Molecular Sciences·Haibo HuangLining Sun
May 29, 2018·Current Cardiology Reports·Arne A N BruyneelMark Mercola
Jan 1, 2018·Advanced Biosystems·Peter KimDeok-Ho Kim
Dec 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Congwu ChiKunhua Song
Feb 6, 2020·International Journal of Molecular Sciences·Congwu ChiKunhua Song
Feb 1, 2018·Nature Biomedical Engineering·Ivan PushkarskyDino Di Carlo
Jul 18, 2017·Trends in Biotechnology·Amr A Abdeen, Krishanu Saha
Feb 26, 2021·Pflügers Archiv : European journal of physiology·Kalina AndrysiakJózef Dulak
Mar 13, 2021·Advanced Nanobiomed Research·Andrew HouseMurat Guvendiren
Oct 20, 2020·Analytica Chimica Acta·Adil MustafaOzlem Yalcin
Sep 9, 2019·ACS Biomaterials Science & Engineering·Zhengnan ZhouGuoxin Tan
Nov 10, 2021·Journal of Biomedical Materials Research. Part a·Shabnam Asghari NiariMohammad Karimipour
Oct 30, 2021·International Journal of Stem Cells·Yingnan LiaoYan Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.