Micropropagation of Phalaenopsis Blume

Methods in Molecular Biology
Pinaki SinhaM Lokman Hakim

Abstract

For high frequency regeneration of Phalaenopsis, young leaf segments are cultured on gelrite-gelled (1/2) MS medium supplemented with 2% sucrose, 2.0 mg/L BA, 0.5 mg/L NAA, 10% coconut water (CW), 2 g/L peptone and 1 g/L activated charcoal. Cultures are incubated at 24 +/- 2 degrees C under fluorescence light 50 micromol/m(2)/s for 16 h photoperiod per day. The PLBs (protocorm like bodies) are induced within 12 weeks of culture and are subcultured to proliferate on the fresh nutrient culture medium for 8 weeks. The PLBs clumps are dissected and cultured on (1/2) MS medium containing 2% sucrose, 10% coconut water (CW), 2 g/L peptone, 150 mg/L L-glutamine and 1 g/L activated charcoal. The PLB sections elongate to form shoots and new PLBs are induced from the base within 8 weeks of culture. For plantlets formation the shoots are cultured on (1/2) MS medium supplemented with 2% sucrose, 10% CW, 2 g/L peptone, 1 g/L activated charcoal and 50 g/L banana pulp. The regenerated plants are acclimatized and cultivated in the nursery, where they bloom within 2 years.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.