Microproteome of dentoalveolar tissues

Bone
Cristiane R SalmonFrancisco H Nociti

Abstract

Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues. Using the LCM-LC-MS/MS approach, a total of 243 proteins was identified from all tissues, 193 proteins in DC, 147 in AB, and 135 proteins DE. Ninety proteins (37% of total) were common to all tissues, whereas 52 proteins (21%) were overlapping in only two. Also, 101 (42%) proteins were exclusively detected in DC (60), AB (15), or DE (26). Identification in all tissues of expected ECM proteins including collagen alpha-1(I) chain (COL1A1), collagen alpha-1(XII) chain (COL12A1), biglycan (BGN), asporin (ASPN), lumican (LUM), and fibromodulin (FMOD), served to validate the approach. Principal component analysis (PCA) and hierarchical clustering identified a high degree of similarity in DC and AB proteomes, where...Continue Reading

Citations

Dec 7, 2018·The FEBS Journal·Oliver R B ThomasBlaine R Roberts
Sep 17, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qiliang ZuoYin Xiao
Nov 20, 2020·Journal of Periodontal Research·Priscila A GiovaniKamila R Kantovitz
Feb 7, 2021·Proteomics·Michal AlexovičRémi Longuespée
Feb 17, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Qianmin OuXuefeng Lin

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